Characterization of Metabolites in an Endophytic Fungus Diaporthe fraxini via NMR-based Metabolomics and Cholinesterase Inhibitory Activity

• Published in: Applied biochemistry and microbiology Vol. 59; no. 3; pp. 316 - 322
• Main Authors: Nagarajan, K., Ibrahim, B., Bawadikji, A.A., Khaw, K.-Y., Tong, W.-Y., Leong, C.-R., Ramanathan, S., Tan, W.-N.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.06.2023; Springer Nature B.V
• Subjects: Acetylcholinesterase; Bioactive compounds; Biochemistry; Biological properties; Biomedical and Life Sciences; Cholinesterase; Cholinesterase inhibitors; Diaporthe; Endophytes; Fungi; Growth conditions; Host plants; Life Sciences; Magnetic properties; Medical Microbiology; Metabolites; Metabolomics; Microbiology; Multivariate analysis; NMR; Nuclear magnetic resonance; Principal components analysis; Diaporthe fraxini; proton nuclear magnetic resonance; cholinesterase; metabolomics; multivariate analysis; endophytes
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683823030134

Endophytic Diaporthe is a fungal genus having an extensive distribution in plant hosts. It is known as a valuable source of bioactive metabolites with potent biological properties. In the present study, proton nuclear magnetic resonance ( 1 H-NMR) coupled with multivariate analysis (MVA) was employed to discriminate the chemical profile of Diaporthe fraxini cultured under different growth conditions. Cholinesterase inhibitory assay was performed to assess the activity of the fungal extracts against acetylcholinesterase and butyrylcholinesterase. Discriminant metabolites responsible for the chemical variations were successfully obtained using 1 H-NMR-based metabolomics approach. Principal component analysis showed a clear discrimination of the fungal extracts of D. fraxini grown under different conditions. Cholinesterase inhibitory activity studies revealed the potential of supplemented cultured fungal extract of D. fraxini as a source of cholinesterase inhibitor.