Coexpression of Vimentin and Cytokeratins in Human Melanoma Cell Lines

—Molecular heterogeneity in seven human metastatic melanoma cells lines is revealed using a double immunofluorescent assay by flow cytometry. It is evaluated that quite a small population of cells (up to 10%) expressed only vimentin, the main marker of melanoma. Only epithelial cytokeratins expressi...

Full description

Saved in:
Bibliographic Details
Published inMoscow University chemistry bulletin Vol. 74; no. 6; pp. 296 - 299
Main Authors Bogush, T. A., Kaliuzhny, S. A., Basharina, A. A., Bogush, E. A., Kirsanov, V. Yu, Kosorukov, V. S., Burova, O. S., Davydov, M. M., Baryshnikova, M. A.
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 01.11.2019
Springer Nature B.V
Subjects
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Flow cytometry
Markers
Melanoma
Metastasis
melanoma
flow cytometry
vimentin
cytokeratins
immunofluorescence assay
Online AccessGet full text

Cover

More Information
Summary:—Molecular heterogeneity in seven human metastatic melanoma cells lines is revealed using a double immunofluorescent assay by flow cytometry. It is evaluated that quite a small population of cells (up to 10%) expressed only vimentin, the main marker of melanoma. Only epithelial cytokeratins expression is revealed in a significantly greater number of cells (from 25 to 50%). The dominant molecular phenotype (50–80% cells expressed both markers), the coexpression of vimentin and cytokeratins, is identified for the first time. The authors assume that the latter phenotype of melanoma cells may be a molecular marker of the tumor’s aggressiveness and metastatic potential.
ISSN:0027-1314
1935-0260
DOI:10.3103/S0027131419060075