OsSGT1 Is a Glucosyltransferase Gene Involved in the Glucose Conjugation of Phenolics in Rice

Phenolics are a class of plant secondary metabolites that play important roles in plant growth and environmental adaptation. Glucosylation of phenolics is one of the molecular mechanisms controlling phenolics homeostasis. However, the relevant glucosyltransferases are largely unknown. In this study,...

Full description

Saved in:
Bibliographic Details
Published inPlant molecular biology reporter Vol. 37; no. 1-2; pp. 41 - 49
Main Authors Liu, Qian, Zhang, Yu-ying, Chen, Lu, Chen, Ting-ting, Li, Yan-jie, Hou, Bing-kai
Format Journal Article
LanguageEnglish
Published New York Springer US 01.04.2019
Springer Nature B.V
Subjects
Adaptation
Bioinformatics
Biomedical and Life Sciences
Conjugation
Dough
Glucose
Glucosyltransferase
Homeostasis
Homology
Life Sciences
Metabolism
Metabolites
Metabolomics
Molecular modelling
Original Paper
Oryza
Phenolic compounds
Phenols
Phylogeny
Plant Breeding/Biotechnology
Plant growth
Plant Sciences
Proteins
Proteomics
Rice
Secondary metabolites
Glycosylation
Enzyme activity
Expression pattern
Phenolics
Glucosyltransferase
Online AccessGet full text

Cover

More Information
Summary:Phenolics are a class of plant secondary metabolites that play important roles in plant growth and environmental adaptation. Glucosylation of phenolics is one of the molecular mechanisms controlling phenolics homeostasis. However, the relevant glucosyltransferases are largely unknown. In this study, a putative family 1 glucosyltransferase gene OsSGT1 was cloned from rice due to its close homology with the previously reported phenolics-related glucosyltransferases UGT84A1-A4, and the phylogenetic relationship of OsSGT1 with homologs from other species was investigated. Recombinant OsSGT1 protein showed strong activity towards phenolics to form their glucose conjugates. This is the first identified natural phenolics-related glucosyltransferase in rice. In addition, the expression patterns of OsSGT1 in different tissues of rice indicated that OsSGT1 was predominantly expressed in the old leaves and dough grains, suggesting that OsSGT1 might be involved in the maturation process of rice by regulating phenolic metabolism, and thus deepened our understanding on the roles of phenolics in rice growth and environmental adaptation.
ISSN:0735-9640
1572-9818
DOI:10.1007/s11105-019-1134-2