Thermo-sensitive Porous Polymer Membrane-immobilized Cellulose as a Switchable Enzyme Reactor for Tuning Its Enzymolysis via Variation Temperature

• Published in: Chemical research in Chinese universities Vol. 39; no. 6; pp. 992 - 997
• Main Authors: Qiao, Juan, Zhang, Xinya, Cheng, Cheng, Qi, Li
• Format: Journal Article
• Language: English
• Published: Changchun Jilin University and The Editorial Department of Chemical Research in Chinese Universities 01.12.2023; Springer Nature B.V
• Subjects: Analytical Chemistry; Carboxymethyl cellulose; Catalytic activity; Cellulase; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; High temperature; Inorganic Chemistry; Maleic anhydride; Membranes; Organic Chemistry; Physical Chemistry; Polymers; Polystyrene resins; Reactors; Substrates; Catalytic efficiency; Plant leaf; Cellulase reactor; Glucose; Thermo-sensitive porous polymer membrane
• ISSN: 1005-9040; 2210-3171
• DOI: 10.1007/s40242-023-3026-0

I mmobilization of enzymes onto porous membranes has attracted considerable attention in recent years. However, enhancing the enzymolysis efficiency of the resulting enzyme reactors by varying the environmental conditions poses a great challenge. In this work, poly(styrene-maleic anhydride- N , N -dimethylacrylamide) was prepared and utilized to construct a thermo-sensitive porous polymer membrane-based enzyme reactor(TS-PPMER) after cellulase was immobilized onto the support by covalent bonding. The catalytic activity of the nano-reactor was evaluated by measuring the yield of the product, glucose, at different temperatures with carboxymethylcellulose as the substrate. Interestingly, the polymer chains coiled and formed numerous nano-pores at a high temperature, which induced the confine effect and greatly boosted the enzymolysis efficiency of TS-PPMER. Furthermore, the proposed TS-PPMER was applied in the hydrolysis of green plant leaves in Epipremnum aureum . This work shows great potential in obtaining biological resources by an environmentally friendly approach using smart polymer-based nano-reactors.