Association of nicotinamide adenine dinucleotide phosphate oxidase p22phox gene 549C〉T polymorphism with coronary artery disease

• Published in: Chinese medical journal Vol. 125; no. 8; pp. 1416 - 1419
• Main Authors: Liu, Tong-tao, Wang, Li-li, Fang, Sheng-xia, Jia, Chong-qi
• Format: Journal Article
• Language: English
• Published: China Department of Cardiology, Qilu Hospital, Shandong University,Jinan, Shandong 250012, China%Department of Epidemiology and Health Statistics, Shandong University, Jinan, Shandong 250012, China 01.04.2012; Dongying Center for Disease Control and Prevention, Dongying,Shandong 257091, China%Department of Epidemiology and Health Statistics, Shandong University, Jinan, Shandong 250012, China
• Subjects: Case-Control Studies; Coronary Artery Disease - etiology; Coronary Artery Disease - genetics; Female; Genotype; Hardy-Weinberg平衡; Humans; Logistic Models; Logistic回归模型; Male; Middle Aged; NADPH Oxidases - genetics; Polymorphism, Single Nucleotide; 冠心病; 基因型频率; 基因多态性; 氧化酶; 烟酰胺腺嘌呤二核苷酸; 焦磷酸测序; coronary artery disease; p22phox; polymorphism; nicotinamide adenine dinucleotide phosphate oxidase; oxidative stress
• ISSN: 0366-6999; 2542-5641
• DOI: 10.3760/cma.j.issn.0366-6999.2012.08.010

Background The p22phox is a critical component of the superoxide-generating vascular nicotinamide adenine dinucleotide phosphate （NADPH） oxidase. Several polymorphisms in p22phox gene are studied for their association with cardiovascular diseases. However, no publication is available to assess the relation of 549C〉T polymorphism in p22pho~ gene to coronary artery disease （CAD） risk. This study was to investigate the effect of the p22phox gene 549C〉T polymorphism on CAD risk. Methods Hospital-based case-control study was conducted with 297 CAD patients and 343 healthy persons as the control group. Polymerase chain reaction and pyrosequencing using PSQ 96 MA Pyrosequencer （Biotage AB） were used to detect the polymorphisms. Multiple Logistic regression model was used to adjust the potential confounders and to estimate odds ratio （OR） with 95% confidence intervals （CIs）. Results The observed genotype frequencies of this polymorphism obeyed the Hardy-Weinberg equilibrium in both cases （P=0.439） and controls （P=-0.668）. The frequency of mutant genotypes （＇I-I-＋CT） in cases （41.08%） was higher than that in controls （36.73%） with an OR=1.20 （95% C1=0.87-1.65）. After the adjustment of the potential confounders, there was a significant association of the mutant genotypes with increased risk of CAD （OR=1.57, 95% C1=1.01-2.46, P=0.047）. Conclusions The mutant genotypes of the p22phox gene 549C〉T polymorphism had a significant effect on the increased risk of CAD in this studied population.