Functional evidence for involvement of bumetanide-sensitive Na +K +2Cl − cotransport in the hepatoportal Na + receptor of the Sprague–Dawley rat

• Published in: Neuroscience letters Vol. 264; no. 1; pp. 65 - 68
• Main Authors: Morita, Hironobu, Fujiki, Nobuhiro, Hagiike, Masanobu, Yamaguchi, Osamu, Lee, Ken
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 02.04.1999; Elsevier
• Subjects: 4-Acetamido-4′-isothiocyanato-stilbene-2,2′-disulfonic acid; Amiloride; Biological and medical sciences; Chlorothiazide; Fundamental and applied biological sciences. Psychology; Furosemide; Hepatic nerve; Hypertonic NaCl; Isolated neuron and nerve. Neuroglia; Ouabain; Vertebrates: nervous system and sense organs; Amiloride; 4-Acetamido-4′-isothiocyanato-stilbene-2,2′-disulfonic acid; Ouabain; Hepatic nerve; Hypertonic NaCl; Furosemide; Chlorothiazide; Vertebrata; Mammalia; Rat; Sodium; Chlorides; Rodentia; Ion transport; Coupled transport; Potassium
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/S0304-3940(99)00157-3

To investigate mechanisms involved in hepatoportal Na + sensing, responses of hepatic afferent nerve activity (HANA) to intraportal hypertonic NaCl injection were measured before, and after, intraportal infusion of inhibitors of Na + transport systems. HANA increased in response to the intraportal injection of 0.75 M NaCl in a dose-dependent manner. The HANA response was not affected by amiloride or 4-acetamido-4′-isothiocyanato-stilbene-2,2′-disulfonic acid (SITS), but was suppressed in a dose-dependent manner by intraportal infusion of ouabain, furosemide, or bumetanide. These results indicate that the hepatoportal Na + receptor senses the Na + concentration via the bumetanide-sensitive Na +K +2Cl − cotransporter.