Plasma Membranes of Rat Liver: Isolation of Lipoprotein Macromolecules

Three high-density lipoprotein classes and one protein were separated from rat-liver plasma membranes that had been treated with mild sonic oscillation. The lipoproteins were separated and identified by techniques in which ultracentrifugation was used. Enzyme markers and electron-microscopic examina...

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Published inScience (American Association for the Advancement of Science) Vol. 156; no. 3775; pp. 665 - 667
Main Authors Barclay, M., Barclay, R. K., Essner, E. S., Skipski, V. P., Terebus-Kekish, O.
Format Journal Article
LanguageEnglish
Published United States American Association for the Advancement of Science 05.05.1967
Subjects
Adenosine Triphosphatases - metabolism
Animals
Cell membranes
Endoplasmic Reticulum - analysis
Erythrocyte membrane
In Vitro Techniques
Lipids
Lipoproteins
Lipoproteins - analysis
Liver
Liver - cytology
Magnesium
Membranes - analysis
Membranes - enzymology
Microscopy, Electron
Mitochondria
Oxidases
P branes
Plasma density
Potassium
Proteins
Rats
Sodium
Ultracentrifugation
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Summary:Three high-density lipoprotein classes and one protein were separated from rat-liver plasma membranes that had been treated with mild sonic oscillation. The lipoproteins were separated and identified by techniques in which ultracentrifugation was used. Enzyme markers and electron-microscopic examination revealed membrane preparations essentially free of contaminating cellular particulates.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0036-8075
1095-9203
DOI:10.1126/science.156.3775.665