Development of 42 SNP markers for the Chinese mitten crab Eriocheir sinensis based on transcriptomics

The Chinese mitten crab ( Eriocheir sinensis ) is one of the most important aquaculture species in China. To discover single nucleotide polymorphisms (SNP) markers for this species, 45 samples were used as templates for PCR validation, and 42 SNP markers were developed in this study. The results sho...

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Published inConservation genetics resources Vol. 9; no. 3; pp. 375 - 377
Main Authors Xiao, Qizhen, Liu, Qing, Dong, Pengsheng, Wu, Xugan, Cheng, Yongxu
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Netherlands 01.09.2017
Springer Nature B.V
Subjects
Animal Genetics and Genomics
Aquaculture
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Ecology
Eriocheir sinensis
Evolutionary Biology
Gene frequency
Genetic diversity
Heterozygosity
Life Sciences
Plant Genetics and Genomics
Single-nucleotide polymorphism
Technical Note
SNP
Transcriptome
Molecular marker
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Summary:The Chinese mitten crab ( Eriocheir sinensis ) is one of the most important aquaculture species in China. To discover single nucleotide polymorphisms (SNP) markers for this species, 45 samples were used as templates for PCR validation, and 42 SNP markers were developed in this study. The results showed that the minor allele frequency ranged from 0.0111 to 0.4778, and the observed heterozygosity ( Ho ) and expected heterozygosity ( He ) varied from 0.0222 to 0.6000 and 0.1061 to 0.5461, respectively. Among these SNPs, seven loci deviated significantly from the Hardy–Weinberg equilibrium ( P  < 0.05). These SNPs will facilitate the assessment of genetic diversity and selective breeding programs for E. sinensis .
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-017-0687-3