Curcumin-loaded hydroxyapatite nanocomposite as a novel biocompatible shield for male Wistar rats from γ-irradiation hazard

• Published in: Chemico-biological interactions Vol. 370; p. 110328
• Main Authors: Sobeh, Eman I., El-ghannam, Gamal, Korany, Reda M.S., Saleh, H.M., Elfeky, Souad A.
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 25.01.2023
• Subjects: Animals; Anti-Inflammatory Agents; Curcumin; Curcumin - chemistry; Curcumin - pharmacology; Durapatite; Glutathione; Hydroxyapatite; Immunohistochemistry; Interleukin-6; Male; Nanocomposites - chemistry; Nanoparticles - chemistry; Rats; Rats, Wistar; Tumor necrosis factor-alpha; Immunohistochemistry; Tumor necrosis factor-alpha; Curcumin; Hydroxyapatite; Interleukin-6; Glutathione
• ISSN: 0009-2797; 1872-7786
• DOI: 10.1016/j.cbi.2022.110328

Curcumin (CUR) is well known for its extraordinary benefits as an anti-cancer, anti-inflammatory, and wound healing agent. However, nano-formulation could maintain and regulate its pharmacological effect. Herein, we report the preparation of CUR/hydroxyapatite nanocomposite (CUR/HA NC) and its application in the protection of male Wistar rats from γ-irradiation carcinogenic consequences. TEM images of the nanocrystalline HA nanoparticles (NPs) had a rod-like form with average dimensions of 40±5 nm in length and 10 ± 5 nm in width. XRD analysis illustrated the formation of a single phase of hexagonal crystalline HA NPs. The presence of the CUR fingerprint is visible in its FTIR spectra of the CUR/HA NC. Biochemical analysis and histological examinations revealed that CUR/HA NC injection does not significantly affect non-irradiation rats compared to the control. However, when injected pre-irradiation, it controls the pro-inflammatory cytokines (tumor necrosis factor-alpha, interleukin-6) GSH level, kidney, and liver functions as proved by biochemical histopathological and immunohistochemical findings. This research introduces a novel effective protection modality for the γ-irradiation hazard via biocompatible CUR/HA NC injection. [Display omitted] •CUR/HA NC was prepared and IP injected in male Wistar rats for shielding from γ-ray.•CUR/HA NC administration at 15 mg/kg pre-irradiation protects rats liver and kidney.•CUR/HA NC controls caspase 3, TNF-α, IL-6, MDA, and GSH levels in γ-irradiated rats.•CUR/HA NC shows a zero-lesion score in liver and kidney of non-irradiated rats.•CUR/HA NC protection against γ-ray occurs by quenching free radicals.