Optimization of core phases of biolistic transformation of potato

• Published in: BIO Web of Conferences Vol. 23; p. 1007
• Main Authors: Malakhova, Natalya P., Skiba, Yuriy A., Tezekbayeva, Botakoz K., Maltseva, Elina R., Ismagulova, Gulnara A.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Les Ulis EDP Sciences 2020
• Subjects: Bombardment; Callus; Cultivation; Explants; Exposure; Genetic transformation; Optimization; Potatoes; Zeatin
• ISSN: 2117-4458; 2273-1709; 2117-4458
• DOI: 10.1051/bioconf/20202301007

We have established the optimal exposure time for internodes and callus plant material of Aksor potato variety on the OSS osmotic medium before and after biolistics and the type of plant explant that is most suitable for that. The optimal objects were explants from internodes of test plants. The best time for exposure of plant material to the osmotic medium before bombardment is 24 hours, and after biolistics – 48 hours. Callus tissue developed on the MS medium with zeatin two weeks later in internodes that were cultivated before bombarding on the OSS for 24 hours and we observed an increase in the callus mass by the end of fourth week. Not a single callus was obtained with rhizogenesis on the R4 medium from internodes and potato calli that were on the osmotic medium within 24 and 72 hours after biolistics. The regenerative capacity of internodes after cultivation on an osmotic medium for 48 hours after bombarding was higher than that of calli. We obtained 12 regenerant plants out of 200 internode explants and 4 regenerant plants out of 150 calli that were used in the experiment.