Identification and Expression Profile of a Neuropeptide LFRFamide-Like Gene During Different Stages of Gonadal Development in the Cephalopod Sepia pharaonis
Neuropeptides are widely distributed in vertebrates and invertebrates, regulating a variety of physiological activities in the organisms, such as metabolism, feeding and reproduction. In this study, to explore the function of neuropeptide LFRFamide in Sepia pharaonis , the full-length cDNA of LFRFam...
Saved in:
Published in | Journal of Ocean University of China Vol. 23; no. 2; pp. 499 - 508 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Heidelberg
Science Press
01.04.2024
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Neuropeptides are widely distributed in vertebrates and invertebrates, regulating a variety of physiological activities in the organisms, such as metabolism, feeding and reproduction. In this study, to explore the function of neuropeptide LFRFamide in
Sepia pharaonis
, the full-length cDNA of
LFRFamide-like
gene (named
SpLFRFL
, MG869822.1) was identified with rapid amplification of cDNA ends (RACE) method. The sequence of
SpLFRFL
was 860 bp in length and encoded 188 amino acids containing 4 different mature peptides: 1 copy of PHTPFRFamide, NSLFRFamide, TIFRFamide, and 3 copies of GNLFRFamide. Multiple alignment and phylogenetic analysis results showed that
Sp
LFRFL shared high identity with LFRFamides of
Sepia officinalis
and
Sepiella japonica
and had the closest relationship with them. Through quantitative Real-time PCR (qRT-PCR), it was found that the
SpLFRFL
gene was highly expressed in the optic lobe and brain at three different stages during gonad development in both genders. Moreover, the four mature peptides at a concentration of 0.01 µmol L
−1
could inhibit the protein synthesis in the Chinese hamster ovary cell strain-K1 (CHO-K1) induced by
Sp
GnRH. These data suggest that
SpLFRFL
might be involved in the development and reproduction of
S. pharaonis
. The results can contribute to future studies on neuropeptide evolution and function and benefit the cuttlefish farming. |
---|---|
ISSN: | 1672-5182 1993-5021 1672-5174 |
DOI: | 10.1007/s11802-024-5715-9 |