Electrophoretic Determination of Carbohydrates in Samples of Natural Origin by an Indirect Detection Method

• Published in: Journal of analytical chemistry (New York, N.Y.) Vol. 79; no. 2; pp. 224 - 232
• Main Authors: Kolobova, E. A., Maliushevska, A. V., Kartsova, L. A.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.02.2024; Springer Nature B.V
• Subjects: Acetic acid; Additives; Analytical Chemistry; Blood plasma; Buckwheat; Carbohydrates; Cetyltrimethylammonium bromide; Chemistry; Chemistry and Materials Science; Chlorides; Electrophoresis; Folic acid; Ionic liquids; carbohydrates; indirect detection; absorbing additives; capillary electrophoresis
• ISSN: 1061-9348; 1608-3199
• DOI: 10.1134/S1061934824020102

Versions of the electrophoretic determination of neutral carbohydrates by a method of indirect detection using acridone acetic and folic acids as absorbing additives ( AA ) are proposed. The effects of the nature and concentration of AA, alkali, and various modifiers (cetyltrimethylammonium bromide, CTAB ) and ionic liquids (1-dodecyl-3-methylimidazolium chloride and 1-hexadecyl-3-methylimidazolium chloride) on the electrophoretic parameters of the migration of analytes are studied. The lowest limits of detection for carbohydrates are achieved in a background electrolyte containing 2.5 mM acridone acetic acid, 75 mM KOH, 0.5 mM CTAB, and 5 vol % MeOH, and amount to 4−10 μg/mL with an efficiency of up to 350 thousand t.p. Under the conditions found, samples of buckwheat honey and human blood plasma are analyzed. It is found that in analyzing food products, it is preferable to use folic acid as a AA, because it provides the best selectivity for the separation of carbohydrates: for the sucralose−sucrose pair, the resolution factor is 7.6.