Liquid chromatographic determination of five benzoylurea insecticides in fruit and vegetables

• Published in: Journal of AOAC International Vol. 81; no. 5; p. 1037
• Main Authors: Gamon, M. (Generalitat Valenciana, Valencia, Spain.), Saez, A, Pelegri, R, Peris, I, Cuadra, J.G. de la, Coscolla, R
• Format: Journal Article
• Language: English
• Published: England 01.09.1998
• Subjects: ALIMENTOS; ANALISIS CUANTITATIVO; ANALYSE QUANTITATIVE; AUBERGINE; AUBERGINES; Benzimidazoles - analysis; BERENJENA; CAPSICUM; CHITIN SYNTHESIS INHIBITORS; CHROMATOGRAPHIE; CHROMATOGRAPHY; Chromatography, Liquid; CONCOMBRE; CONTAMINACION; CONTAMINATION; CROMATOGRAFIA; CUCUMBERS; DIFLUBENZURON; ENSAYO; EVALUACION; EVALUATION; FLUFENOXURON; Food Contamination; FOODS; Fruit - chemistry; HEXAFLUMURON; INHIBIDORES DE SINTESIS DE QUITINA; INHIBITEUR DE SYNTHESE DE CHITINE; INSECTICIDAS; INSECTICIDE; INSECTICIDES; LIQUID CHROMATOGRAPHY; LUFENURON; MEASUREMENT; MEDICION; MESURE; NARANJA DULCE; ORANGE DOUCE; PEPINO; Pesticide Residues - analysis; PRODUIT ALIMENTAIRE; QUANTITATIVE ANALYSIS; RESIDU; RESIDUES; RESIDUOS; Sensitivity and Specificity; SWEET ORANGES; TEFLUBENZURON; TEST PROCEDURE; TESTAGE; TESTING; Time Factors; TOMATE; TOMATOES; Urea - chemistry; Vegetables - chemistry
• ISSN: 1060-3271; 1944-7922
• DOI: 10.1093/jaoac/81.5.1037

A liquid chromatographic (LC) method was developed to determine 5 benzoylureas--diflubenzuron, hexaflumuron, teflubenzuron, flufenozuron, and lufenuron--in peppers, tomatoes, eggplants, cucumbers, and oranges. Preparation of samples involve extraction with acetone and partitioning into dichloromethane-petroleum ether. A portion of this extract is cleaned up with a solid-phase extraction aminopropyl disposable column. With LC analysis using an RP-8-DB microbore column, acetonitrile-water (70 + 30, v/v) as mobile phase, and photodiode array detection at 254 nm, recovery and repeatability data were collected for the 5 benzoylureas on 4 vegetables and citrus in the range 0.04-2.0 mg/kg. Validated limits of detection and quantitation were 0.01 and 0.04 mg/kg, respectively. The method is reliable for routine analysis of vegetables and fruits