Synthesis of [Mn(Salen)Cl] Complex Compound and Superoxide Dismutase Activity Determination through Non-Enzymatic Method

• Published in: Key engineering materials Vol. 811; pp. 22 - 27
• Main Authors: Kurnia, Dikdik, Deawati, Yusi, Utami, Ratna Annisa, Mulyani, Irma, Hastiawan, Iwan, Onggo, Djulia
• Format: Journal Article
• Language: English
• Published: Zurich Trans Tech Publications Ltd 01.07.2019
• Subjects: Complex compounds; Hydrogen peroxide; In vitro methods and tests; Infrared spectra; Ions; Magnetic moments; Magnetic permeability; Manganese; Photochemistry; Riboflavin; Superoxide dismutase; SOD; Dismutase; Superoxide; Mimic; Mn(Salen)Cl; Complex
• ISSN: 1013-9826; 1662-9795; 1662-9795
• DOI: 10.4028/www.scientific.net/KEM.811.22

Superoxide dismutase (SOD) is an Mn-containing enzyme that catalyzes the conversion of superoxide (O2•−) into O2 and H2O2. To develop a small, non-protein Mn-containing SOD mimic, an [Mn(salen)Cl] complex was synthesized from MnCl2.4H2O and H2salen. The product yielded 75-90% reddish dark brown crystals indicative of the presence of manganese(III) metal ions. An elemental analysis showed that the compound contained: C = 48.36%; H = 4.34%; and N = 7.13%. Infrared spectra of this compound showed peaks at: υ(OH)=3426 (medium), υ(C=N)=1634 (very high), υ(CO)=1292 (high). An ESI-MS analysis (m/z) showed the presence of ionic species from the complex including (salen)(H+) = 269.0 and [Mn(salen)]+ = 320.9. Conductivity measurements revealed that the compound is neutral with a molar conductivity of 0.17 mS. Its magnetic moment of 5.2 BM was obtained from a magnetic susceptibility balance measurement, showing that the compound is paramagnetic. The SOD activity of this compound was given by IC50 = 2.5 ± 0.3 µM in a non-enzymatic riboflavin photoreduction in vitro test.