320 Oxygenated Peritoneal Perfluorodecalin Improves Response to Normobaric Hypoxic Exposure in Swine

• Published in: Journal of clinical and translational science Vol. 7; no. s1; p. 96
• Main Authors: Leibowitz, Joshua L, Awad, Morcos A., Stachnik, Stephen, Moon, Yejin, Kadkhodaeielyaderani, Behzad, Hahn, Jin-Oh, Fathy, Hosam K., Stewart, Shelby J., Friedberg, Joseph S.
• Format: Journal Article
• Language: English
• Published: Cambridge Cambridge University Press 01.04.2023
• Subjects: Abdomen; Animal models; Blood; Carbon dioxide; Clinical trials; Gas exchange; Hypercapnia; Hypoventilation; Hypoxia; Mechanical ventilation; Oxygen; Perfluorocarbons; Peritoneum; Population studies; Precision Medicine/Health; Survival
• ISSN: 2059-8661; 2059-8661
• DOI: 10.1017/cts.2023.371

OBJECTIVES/GOALS: Patients suffering from respiratory failure have few options to support oxygenation and carbon dioxide removal aside from mechanical ventilation. Our objective was to test a novel extrapulmonary mechanism of gas exchange via peritoneal oxygenated perfluorocarbon (PFC) in a large animal model. METHODS/STUDY POPULATION: Using two 50 kg swine, hypoxia was modeled with subatmospheric oxygen and hypercarbia induced with acute hypoventilation. Through a midline laparotomy, cannulas were placed into the peritoneal space to allow for PFC infusion and circulation. After abdominal closure, these cannulas were connected to a device capable of draining, oxygenating, and infusing PFC. One animal was subjected to acute hypoxia (12% FiO2) and another animal to acute hypoventilation (4 breaths per minute). Primary outcomes were times for SpO2 to reach 75 mmHg, respectively. Trials were performed without PFC and with PFC dwelling or circulating through the peritoneal space, during which abdominal and bladder pressures were monitored and maintained under 20 mmHg by regulation of the PFC volume contained in the animal. RESULTS/ANTICIPATED RESULTS: In the animal subjected to acute hypoxia (12% FiO2), survival time improved from 5:55 to 20:00 (min:sec) after 2.5 liters of oxygenated PFC was instilled in the peritoneal space. Oxygen percent saturation of PFC before and after dwelling in the peritoneal space was measured at 100% before and 70% after dwelling in the animal during this hypoxic period corresponding with a gas transfer of 300 mL of oxygen over the 20-minute trial (i.e., 15 mL/min). Continual PFC circulation did not further extend the survival time during hypoxic conditions over PFC dwelling in the abdomen. In the animal that was acutely hypoventilated, there were no detectable differences in the rate of CO2 accumulation as measured by EtCO2 or direct blood pCO2 measurements with PFC dwelling or circulating through the peritoneal space. DISCUSSION/SIGNIFICANCE: Oxygenated PFC dwelling in the peritoneal space increased the duration of systemic arterial blood saturation remaining greater than 50% during normobaric hypoxic (12% FiO2) conditions but did not appreciably clear blood carbon dioxide during hypoventilation. Future experiments will focus on maximizing the rate of systemic oxygen uptake.