Stenotrophomonas cyclobalanopsidis sp. nov., isolated from the leaf spot disease of Cyclobalanopsis patelliformis

• Published in: Antonie van Leeuwenhoek Vol. 113; no. 10; pp. 1447 - 1454
• Main Authors: Bian, Dan-ran, Xue, Han, Piao, Chun-gen, Li, Yong
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.10.2020; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Cyclobalanopsis; Deoxyribonucleic acid; Diphosphatidylglycerol; DNA; Fatty acids; Gene sequencing; Leafspot; Leaves; Life Sciences; Lipids; Medical Microbiology; Microbiology; New species; Nucleotides; Original Paper; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipids; Phylogeny; Plant Sciences; rRNA 16S; Soil Science & Conservation; Stenotrophomonas; Polyphasic taxonomic; Genome; Average nucleotide identity
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-020-01453-y

A Gram-negative, facultatively anaerobic, motile bacterial strain, TPQG1-4 T , was isolated from the leaf of Cyclobalanopsis patelliformis with spot disease. The isolate was investigated using the polyphasic taxonomic approach. 16S rRNA gene sequencing and analyzing revealed that the novel strain shares the highest sequence similarity with Stenotrophomonas lactitubi M15 T (99.6%), Stenotrophomonas indicatrix WS40 T (99.4%), Stenotrophomonas maltophilia IAM 12423 T (99.2%) and Stenotrophomonas pavanii LMG 25348 T (99.0%). In phylogenetic trees based on 16S rRNA gene sequences, the novel strain branched independently from other species of Stenotrophomonas. Average nucleotide identity values between the novel isolate and S. lactitubi M15 T , S. indicatrix WS40 T , S. maltophilia IAM 12423 T , S. pavanii LMG 25348 T , and Pseudomonas geniculata ATCC 19374 T were 87.2%, 87.3%, 86.3%, 88.0%, and 81.3%, respectively, suggesting the isolate was a novel species of the genus Stenotrophomonas. The DNA G + C content of TPQG1-4 T is 67.1 mol%. The major fatty acids were iso-C 15:0 (25.4%) and anteiso-C 15:0 (17.0%). The polar lipids of TPQG1-4 T included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, amino phospholipid and phospholipid. Based on phenotypic and genotypic characteristics, the strain represents a novel species in the genus Stenotrophomonas , for which the name Stenotrophomonas cyclobalanopsidis sp. nov. is proposed. The type strain is TPQG1-4 T (= CFCC 15341 T  = LMG 31208 T ).