Structural and immunologic evidence for a small molecular weight ("21K") variant of prolactin

The mol wt (Mr) of intact murine PRL is approximately 23,000. Immunoblotting analysis shows a 21,000 Mr band in fresh rat and mouse pituitary extracts that cross-reacts strongly with PRL antibodies. The band becomes markedly altered by stimuli known to influence PRL secretion, such as nursing, estra...

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Bibliographic Details
Published inEndocrinology (Philadelphia) Vol. 123; no. 3; p. 1364
Main Authors Sinha, Y N, Jacobsen, B P
Format Journal Article
LanguageEnglish
Published United States 01.09.1988
Subjects
Animals
Bromocriptine - pharmacology
Estradiol - pharmacology
Female
Genetic Variation
Lactation
Male
Mice
Mice, Inbred Strains
Molecular Weight
Ovariectomy
Peptide Mapping
Perphenazine - pharmacology
Pituitary Gland - drug effects
Pituitary Gland - metabolism
Pregnancy
Progesterone - pharmacology
Prolactin - genetics
Prolactin - immunology
Prolactin - isolation & purification
Rats
Rats, Inbred Strains
Reference Values
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Summary:The mol wt (Mr) of intact murine PRL is approximately 23,000. Immunoblotting analysis shows a 21,000 Mr band in fresh rat and mouse pituitary extracts that cross-reacts strongly with PRL antibodies. The band becomes markedly altered by stimuli known to influence PRL secretion, such as nursing, estradiol benzoate, and perphenazine. By two-dimensional electrophoresis, it splits into at least three components, two of which have more acidic pI than PRL. The tyrosine peptide maps of the three proteins resemble that of PRL. These results indicate that the 21,000 Mr band consists of a cluster of proteins that are structural variants of PRL, raising the possibility that these variants result from alternative splicing of the PRL gene transcript.
ISSN:0013-7227
DOI:10.1210/endo-123-3-1364