Application of dyes as doping agents in MALDI-MS matrices for the signal enhancement of proteins

Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS) is considered as a technique of choice for analysing proteins and peptides. Several modifications have been proposed to increase the sensitivity of MALDI-MS procedure so that low abundant proteins can be targeted. The present w...

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Bibliographic Details
Published inRSC advances Vol. 7; no. 11; pp. 6598 - 6604
Main Authors Ali, Arslan, Shahid, Najia, Musharraf, Syed Ghulam
Format Journal Article
LanguageEnglish
Published 2017
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Summary:Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS) is considered as a technique of choice for analysing proteins and peptides. Several modifications have been proposed to increase the sensitivity of MALDI-MS procedure so that low abundant proteins can be targeted. The present work describes the study of the possible increase in MALDI-MS sensitivity by increasing the ion generation via use of dopants in MALDI-MS matrices. These dopant constitutes commonly used dyes such as Coomassie Blue G-250 (A), Cochineal (B), Erythrosin B (C), Sunset yellow (D), and Acid Red 1 (E), which when employed in 0.1–100 pM range along with conventional MALDI-MS matrices such as α-cyano-4-hydroxycinnamic acid (HCCA), sinapic acid (SA) and 2,5-dihydroxybenzoic acid (DHB), can significantly increase (×2–35) the ion intensity of proteins such as bovine serum albumin (BSA), myoglobin, casein and insulin. Moreover, such effect remains consistent when applied to analyse real samples such as honey and egg white analysis.
ISSN:2046-2069
2046-2069
DOI:10.1039/C6RA27156A