Distribution of Phenotypic and Genotypic Antibiotic Resistance in E. coli Isolates along the Production and Supply Chain of Pork around Hubei Province of China

• Published in: Pakistan journal of zoology Vol. 51; no. 4; p. 1569
• Main Authors: Khan, Sher Bahadar, Geng, Zou, Yu-ting, Cheng, Sultan, Asad, Ali, Mumtaz, Ahmad, Irshad, Zhou, Rui
• Format: Journal Article
• Language: English
• Published: Lahore Knowledge Bylanes 31.08.2019; AsiaNet Pakistan (Pvt) Ltd
• Subjects: Abattoirs; Animal feeding and feeds; Anopheles; Antibiotic resistance; Antibiotics; Antimicrobial agents; Business logistics; Deoxyribonucleic acid; DNA; Drug resistance; E coli; Economic conditions; Escherichia coli; Farms; Feed industry; Health risks; Intestine; Laboratories; Legal fees; Liver; Meat; Microbial drug resistance; Microbiological research; Multidrug resistance; Pathogenic microorganisms; Pathogens; Phylogenetics; Pork; Pork industry; Public health; Supply chains; Swine; Tetracyclines; Thermal cycling; Tonsil; Virulence; China; United States > US
• ISSN: 0030-9923
• DOI: 10.17582/journal.pjz/2019.51.4.1569.1574

ABSTRACT Using MacConkey agar, E. coli were isolated from 285 samples including 125 tonsil swabs (4-6 weeks old healthy pigs from 5 farms), 80 tissue samples from different slaughter houses (20 each intestine, liver, meat and kidney), and 80 samples from super-and wet-markets (each 20 meat and liver) collected both in summer and winter. Isolates were tested for 15 antibiotics (CRO, AMX, GEN, STR, TET, CHL, CLR, LVX, OFX, GAT, CIP, SXT, AMP, LIN and AZM) according to the disc diffusion method and antibiotic resistant genes {tet(A), tet(B), tet(C), strA/strB, aadA, aac(3)IV), aadB, sul1, sul2 and sul3, blaCMY-2, blaTEM and blaSHV} using mPCR. Resistance for LIN was the highest in overall (96.3%, 77/80) isolates as well as from pig farms (100%, 20/20) and different markets (100%, 20/20, 85%, 17/20). Resistance for other antibiotics such as AMX, TET, AMP and SXT was found 82.5% (66/80), 63.7% (51/80), 58.7% (47/80) and 50% (40/80), respectively. The most prevalent ARGs in the isolates recovered from pig farms was blaTEM (100%, 20/20), followed by blaCMY-2 (80%, 16/20), tetA and tetB (60%, 12/20) and tetC (50%, 10/20). E. coli became more and more diverse along the PSCP with group B2 being the most prevalent. Besides multiple drug resistance, they share many traits with the human pathogenic isolates based on virulence gene contents that may pose a potential threat to public health.