Comparison of response mechanism of ordinary Cordyceps militaris and domesticated Cordyceps militaris to Pb2+ stress

[Display omitted] •Cordyceps militaris resisted Pb stress mainly relying on protein glutathione (PB-SH).•The genes of ugp was the key enzyme under the stress of heavy metal.•The domesticated Cordyceps militaris could alleviate oxidative stress caused by Pb2+. In this study, we compared the response...

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Published inProcess biochemistry (1991) Vol. 107; pp. 112 - 120
Main Authors Song, Qiaoying, Li, Yaqi, Jia, Yunqin, Pan, Lichao, Zhu, Zhen-yuan
Format Journal Article
LanguageEnglish
Published Barking Elsevier Ltd 01.08.2021
Elsevier BV
Subjects
Biomass
Cordyceps militaris
Domestication
Enzymatic activity
Enzyme activity
Enzymes
Glutathione
Heavy metal
Heavy metals
Lead
Oxidative stress
Pb2+ stress
Polysaccharides
Proteins
Response mechanism
Pb2+ stress
Response mechanism
Cordyceps militaris
Heavy metal
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Summary:[Display omitted] •Cordyceps militaris resisted Pb stress mainly relying on protein glutathione (PB-SH).•The genes of ugp was the key enzyme under the stress of heavy metal.•The domesticated Cordyceps militaris could alleviate oxidative stress caused by Pb2+. In this study, we compared the response mechanism of ordinary Cordyceps militaris and domesticated one to Pb2+ stress by exploring the reduced glutathione content (GSH), biomass, expression level of enzymes related to polysaccharide and enzyme activity of GSH-PX, SOD and CAT. Results showed that the GSH was mainly concentrated in intracellular, and the content of protein GSH (PBSH) was higher than non-protein GSH (NPSH), which revealed that the Cordyceps militaris resisted heavy metal mainly relying on PBSH. Besides, the biomass level of domesticated group was always higher than that in the ordinary group. In addition, the domesticated group had a higher enzyme activity of PGM, PGI and UGP. And the genes of ugp was the most critical enzyme under the stress of heavy metal. Further more, the domesticated Cordyceps militaris could alleviate the oxidative stress caused by Pb2+, and exercise the function of relieving Pb2+ poisoning. Owing to its preferable response mechanism, domesticated Cordyceps militaris revealed good prospect in removing heavy metals from organisms.
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2021.05.012