Characterization of pig lymphocyte subpopulations by adherence to nylon wool

The property of adherence to nylon wool was used to separate and characterize pig blood lymphocyte subpopulations. Thymus-dependent null cells proved the least adherent lymphocytes, and B cells, bearing surface Ig or Fc receptor, the most adherent. sIg+ lymphocytes detected by immunofluorescence and...

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Bibliographic Details
Published inInternational archives of allergy and applied immunology Vol. 58; no. 2; pp. 128 - 134
Main Authors Binns, R.M, Vaiman, M, Davies, H.S, Symons, D.B.A
Format Journal Article
LanguageEnglish
Published Switzerland 1979
Subjects
animal diseases
animal health
Animals
Antibodies, Anti-Idiotypic
Cell Adhesion
Cell Separation
Cytotoxicity Tests, Immunologic
Histocompatibility Antigens - immunology
Immunoglobulin Fc Fragments
Immunologic Techniques
Lymphocytes - immunology
Nylons
Receptors, Antigen, B-Cell
Rosette Formation
Sheep
Swine
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Summary:The property of adherence to nylon wool was used to separate and characterize pig blood lymphocyte subpopulations. Thymus-dependent null cells proved the least adherent lymphocytes, and B cells, bearing surface Ig or Fc receptor, the most adherent. sIg+ lymphocytes detected by immunofluorescence and by 51Cr release cytotoxicity showed similar frequency and adherence properties. Lymphocytes rosetting with SRBC in PBS or in dextran had similar adherence patterns and showed both non-adherent and adherent components. Lymphocytes bearing major histocompatibility complex (MHC) Ia-like antigens shown by eosin exclusion or 51Cr release cytotoxicity were enriched in the adherent fraction, but also occurred in significant numbers in the non-adherent fraction. Non-adherent Ia+ cells were predominantly sIg- and probably T cells, whereas the adherent Ia+ group included the sIg+ cells and some sIg- cells.
ISSN:0020-5915
DOI:10.1159/000232184