First-Generation Monoclonal Antibodies Identifying the Human Leukotriene B4 Receptor-1

The leukotriene B4 receptor (BLTR) is a seven-transmembrane chemoattractant receptor that is important in pro-inflammatory responses. We have produced the first widely applicable monoclonal antibodies against the human BLTR and confirmed the antibody specificity using flow cytometric analysis of thr...

Full description

Saved in:
Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 279; no. 2; pp. 520 - 525
Main Authors Pettersson, Annika, Boketoft, Åke, Sabirsh, Alan, Nilsson, Niclas E., Kotarsky, Knut, Olde, Björn, Owman, Christer
Format Journal Article
LanguageEnglish
Published Elsevier Inc 01.12.2000
Subjects
BLTR
endogenous receptor
HL-60 cells
leukocytes
leukotriene B4 receptor
monoclonal antibodies
recombinant receptor
endogenous receptor
BLTR
leukocytes
leukotriene B4 receptor
HL-60 cells
recombinant receptor
monoclonal antibodies
Online AccessGet full text

Cover

More Information
Summary:The leukotriene B4 receptor (BLTR) is a seven-transmembrane chemoattractant receptor that is important in pro-inflammatory responses. We have produced the first widely applicable monoclonal antibodies against the human BLTR and confirmed the antibody specificity using flow cytometric analysis of three different cell lines stably expressing the recombinant receptor. The antibodies did not cross-react with the recently cloned second LTB4 receptor, BLTR2, or the Cys LT1 and Cys LT2 receptors. Functional analysis in combination with two-color flow cytometry showed that the BLTR antibodies bind to cells that are activated by LTB4. The antibodies were shown to recognize BLTR in cell ELISA and immunocytochemistry. Endogenous expression of BLTR in CD15-positive blood leukocytes and in differentiated HL-60 cells was also demonstrated with the antibodies.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2000.3989