Genomic analysis of DNA-protein interaction by chromatin immunoprecipitation

Chromatin immunoprecipitation (ChIP) is an effective technique to analyze the interactions of DNA binding proteins with the genome in vivo. ChIP coupled with high density microarray (ChIP-chip) or high-throughput sequencing (ChIP-Seq) has generated large amount of data and expected to allow the deve...

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Bibliographic Details
Published inYíchuán Vol. 32; no. 3; p. 219
Main Authors Li, Min-Li, Wang, Wei, Lu, Zu-Hong
Format Journal Article
LanguageChinese
Published China 01.03.2010
Subjects
Animals
Chromatin Immunoprecipitation
DNA - metabolism
Humans
Protein Binding
Proteins - metabolism
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Summary:Chromatin immunoprecipitation (ChIP) is an effective technique to analyze the interactions of DNA binding proteins with the genome in vivo. ChIP coupled with high density microarray (ChIP-chip) or high-throughput sequencing (ChIP-Seq) has generated large amount of data and expected to allow the development of a network describing the cellular transcriptional regulation. Here, we reviewed the ChIP, ChIP-chip, and ChIP-Seq techniques as well as their perspectives. Focus is given to data analysis of ChIP-Seq and the applications of ChIP-chip and ChIP-Seq.
ISSN:0253-9772
DOI:10.3724/SP.J.1005.2010.00219