Bovine early pregnancy factor: Purification and biochemical examination

The isolation and purification of early pregnancy factor (EPF) in the serum of pregnant bovine is described. The serum of 10.4 1 was obtained from a pregnant bovine of 8 days after artificial insemination. The rosette inhibition titer (RIT) of the serum was 6. EPF was isolated using the diafiltratio...

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Bibliographic Details
Published inJournal of Reproduction and Development Vol. 38; no. 6; pp. j39 - j48
Main Authors Ito, K. (Iwate Univ., Morioka (Japan). Faculty of Agriculture), Yasuda, Y
Format Journal Article
LanguageEnglish
Published THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT 1992
Subjects
BIOCHEMISTRY
BIOCHIMIE
BIOQUIMICA
BLOOD SERUM
BOVINAE
Bovine Serum
EPF
GESTACION
GESTATION
PREGNANCY
PURIFICACION
PURIFICATION
Purification and partial characterization of EPF
RIT
SERUM SANGUIN
SUERO SANGUINEO
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Summary:The isolation and purification of early pregnancy factor (EPF) in the serum of pregnant bovine is described. The serum of 10.4 1 was obtained from a pregnant bovine of 8 days after artificial insemination. The rosette inhibition titer (RIT) of the serum was 6. EPF was isolated using the diafiltration, ion-exchange chromatography (CM-Sepharose, DEAE-Sepharose) and FPLC-gel permeation chromatography. EPF active fraction was recognized in the elute (RIT=7) of 50 mM NaCl on the CM-Sepharose and the unadsorbed fraction (RIT ?? 8) on the DEAE-Sepharose. The unadsorbed fraction of DEAE-Sepharose had 4 bands by SDS-PAGE analysis. The molecular weight of 4 bands were 23, 22, 21.5, 21 KD respectively. Further, the fraction No. 32 of the FPLC had a high EPF activity (RIT ?? 8) and the molecular weight of this fraction was estimated as 2122 KD. The iso-electoric point of EPF was near 5.0 by 2D-SDS-PAGE analysis.
Bibliography:9306495
L53
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.38.6_j39