Non-invasive micro-test technology and applications

• Published in: Biophysics reports Vol. 11; no. 2; pp. 96 - 111
• Main Authors: Sun, Kai, Liu, Yunqi, Pan, Yanshu, Di, Dongwei, Li, Jianfang, Xu, Feiyun, Li, Li, Mimata, Yoshiharu, Chen, Yingying, Xie, Lixia, Wang, Siqi, Qi, Wenqian, Tang, Yan, Sheng, Huachun, Wang, Bing, Sun, Ruixue, Tan, Dingquan, Fu, Daohong, Yin, Ye, Xue, Ao, Shi, Yichao, Shao, Wenjing, Gong, Lei, Jiang, Zhijian, Zhang, Wei, Wu, Qiangsheng, Wang, Yaosheng, Lang, Minglin, Ye, Wenxiu, Xu, Weifeng, Wei, Shuhe, Shi, Weiming, Xu, Yue Jeff
• Format: Journal Article
• Language: English
• Published: China Biophysics Reports Editorial Office 30.04.2025
• Subjects: Review; H+-ATPase; Physiology; Ionic homeostasis; Non-invasive micro-test technology; Transmembrane transport; Calcium signature
• ISSN: 2364-3439; 2364-3420; 2364-3420
• DOI: 10.52601/bpr.2024.240009

Non-invasive micro-test technology (NMT) reveals dynamic ionic/molecular concentration gradients by measuring fluxes of ions and small molecules in liquid media in 1D, 2D or 3D fashions with sensitivity up to pico- (10 ) or femto- (10 ) moles per cm per second. NMT has been applied to study metabolism, signal transduction, genes and/or proteins physiological functions related to transmembrane ionic/molecular activities with live samples under normal conditions or stress. Data on ion and/or molecule homeostasis (IMH) by NMT in biomedical sciences, plant and crop sciences, environmental sciences, marine and space biology as well as traditional Chinese medicine are reviewed.