Adhesion and colonization of potential probiont Pseudomonas aeruginosa FARP72 in the intestine of yellowtail catfish, Pangasius pangasius

• Published in: Archives of microbiology Vol. 203; no. 5; pp. 2711 - 2717
• Main Authors: Hoque, Farhana, Abraham, T. Jawahar
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.07.2021; Springer Nature B.V
• Subjects: Adhesion; Aquaculture; Archives & records; Bacteria; Binding sites; Biochemistry; Biomedical and Life Sciences; Biotechnology; Catfish; Cell Biology; Colonization; Ecology; Electron micrographs; Epidemics; Fishery sciences; Intestine; Laboratory animals; Life Sciences; Microbial Ecology; Microbiology; Pangasius pangasius; Pathogens; Physiology; Probiotics; Pseudomonas; Pseudomonas aeruginosa; Salmonella; Short Communication; Sodium chloride; Tubes; Yellowtail; Catfish aquaculture; Probiont; Antagonistic bacteria; Adhesion
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-021-02188-8

Adhesion is recognized as the first important step of a probiont for intestinal colonization. This study assessed the ability of an antagonistic Pseudomonas aeruginosa FARP 72 to adhere and colonize the intestine of yellowtail catfish, Pangasius pangasius both in vitro and in vivo. For the in vitro assay, the whole intestines of each of two starved P. pangasius were introduced separately into tubes containing bluish-green pigment-producing P. aeruginosa FARP 72 at 8.00 log 10 CFU/mL and physiological saline (0.85% sodium chloride) and incubated for 1 h at 30 ± 1 °C. The homogenate mucus solutions from the intestine samples were serially diluted and plated onto Pseudomonas isolation agar to determine the counts of bluish-green pigment-producing P. aeruginosa (BPPAC). The difference between the numbers of BPPAC and presumptive Pseudomonas counts (PPC) in the treated and control intestines was attributed to the adherence of P. aeruginosa FARP 72 . The levels of BPPAC and PPC in the treated intestines were 6.09 ± 0.59 log 10 CFU/g. Similarly, following 30 days of feeding P. pangasius with P. aeruginosa FARP 72 supplemented diet, the intestine of catfish recorded the BPPAC of 5.83 ± 0.64 log CFU/g. In control samples, the BPPACs were recorded as < 3.00 log 10 CFU/g. The scanning electron micrograph of the intestines of P. pangasius following the in vitro and in vivo adhesion assays confirmed the ability of this bacterium to strongly adhere to the intestine, thus making it most suitable candidate probiont for use in freshwater catfish aquaculture.