Production and Purification of Antioxidant Peptides from Flatfish Skin Protein Hydrolysates

• Published in: Transactions of Tianjin University Vol. 21; no. 5; pp. 433 - 439
• Main Authors: Zhu, Hongji, Wang, Shipeng, Tian, Li, Zhang, Hua
• Format: Journal Article
• Language: English
• Published: Tianjin Tianjin University 01.10.2015
• Subjects: DPPH自由基; Engineering; Humanities and Social Sciences; Mechanical Engineering; multidisciplinary; Science; 分离; 反相高效液相色谱法; 抗氧化肽; 比目鱼皮; 皮肤; 自由基清除能力; 蛋白水解物; antioxidant peptides; optimization; enzymatic hydrolysis; purification; flatfish skin
• ISSN: 1006-4982; 1995-8196
• DOI: 10.1007/s12209-015-2432-x

Antioxidant peptides of flatfish skin protein hydrolyzed by four enzymes （Papain, Pepsin, Trypsin and Neutrase, respectively）were investigated. The Trypsin hydrotysate obtained by hydrolysis exhibited the highest 1,1- dipheny-l-2-picrylhydrazyl（DPPH） radical scavenging activity （DRSA） compared with other hydrolysates. Re- sponse surface method ology （RSM）, based on Box-Behnken design, was used to study the influence of hydrolysis conditions on the DRSA. The optimal hydrolysis conditions were as follows： pH 7.38, temperature 48.2~C and en- zyme/substrate （E/S）ratio 2 840 U/g. Under these conditions, the maximum DRSA was （22.85 ＋ 0.57）%. The experimental values agreed with the value （23.09%） predicted by the model within a 95% confidence interval. By using gel filtration chromatography and reversed-phase high performance liquid chromatography （RP-HPLC）, anti- oxidant peptide （D2-P）was isolated from flatfish skin protein hydrolysates （FSPH）and could exhibit a （54.28 ± 1.37） % scavenging activity on DPPH radical at the concentration of 5 mg/mL. This is the first report of a scientific basis for the preparation of antioxidant peptides from flatfish skin. The results suggested that the antioxidant pep- tides can be exploited into functional foods or used as a novel source of nutraceuticals.