Intracellular calcium imaging for agonist screening

Calcium ions are involved in the regulation of a wide range of physiological activities such as nerve conduction, muscle contraction, cell division and gene expression through local calcium transients and global calcium oscillations. Calcium homeostasis modulator 1 (CALHM1) is a novel plasma membran...

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Published inBiophysics reports Vol. 11; no. 3; pp. 164 - 171
Main Authors Wang, Haojie, Yang, Bo, Mo, Liangzhu, Yang, Hua-Qian
Format Journal Article
LanguageEnglish
Published China Biophysics Reports Editorial Office 30.06.2025
Subjects
Protocol
Fluo-8
Calcium imaging
Calcium homeostasis modulator 1
jGCaMP8m
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Summary:Calcium ions are involved in the regulation of a wide range of physiological activities such as nerve conduction, muscle contraction, cell division and gene expression through local calcium transients and global calcium oscillations. Calcium homeostasis modulator 1 (CALHM1) is a novel plasma membrane large-pore ion channel mediating calcium influx. Therefore, screening for novel CALHM1 agonists or antagonists is very important for the research of physiological and pathological processes and the development of therapeutic drugs for related diseases. In this protocol, we presented the detection of real-time calcium dynamics in transiently transfected cell lines and primary cells with genetically encoded calcium indicators or calcium indicator dyes, respectively. A comprehensive step-by-step approach has been outlined for drug screening and validation to offer a valuable guide for utilizing calcium imaging to investigate calcium-related physiological processes.
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Haojie Wang, Bo Yang and Liangzhu Mo contributed equally to this work.
ISSN:2364-3439
2364-3420
2364-3420
DOI:10.52601/bpr.2024.240026