Artemisia capillaris Thunb. inhibits melanin synthesis activity via ERK-dependent MITF pathway in B16/F10 melanoma cells

Genus Artemisia occurs as a hardy plant and has a wide range of culinary and medicinal features. In thisstudy, we aimed to describe the melanin inhibitory activity of one Artemisia species, i.e., Artemisia capillaris Thunb. Ethanol extracts of fermented Artemisia capillaris (Art.EtOH.FT) and non-fer...

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Published inKorean journal of veterinary research Vol. 58; no. 1; pp. 1 - 7
Main Authors Saba, Evelyn, Oh, Mi Ju, Lee, Yuan Yee, Kwak, Dongmi, Kim, Suk, Rhee, Man Hee
Format Journal Article
LanguageEnglish
Published 대한수의학회 2018
Subjects
수의학
hypopigmentation
Artemisia capillaris
tyrosinase
melanin inhibition
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Summary:Genus Artemisia occurs as a hardy plant and has a wide range of culinary and medicinal features. In thisstudy, we aimed to describe the melanin inhibitory activity of one Artemisia species, i.e., Artemisia capillaris Thunb. Ethanol extracts of fermented Artemisia capillaris (Art.EtOH.FT) and non-fermented Artemisia capillaris (Art.EtOH.CT)were tested for their ability to inhibit tyrosinase activity and melanin pigmentation. Both extracts showed dose-dependentinhibition against α-melanocyte stimulating hormone−stimulated melanin formation and tyrosinase activity, withoutcytotoxicity. At 100 μg/mL, both extracts showed greater inhibition than kojic acid, the positive control. Proteinexpressions of microphthalmia-associated transcription factor (MITF), tyrosinase (TYR), tyrosinase-related protein 1(TRP-1), and tyrosinase-related protein 2 (TRP-2) at the transcriptional level were determined by using real-time andsemi-quantitative polymerase chain reaction. To complete the mechanistic study, presences of upstream elements ofMITF, the phosphorylated-extracellular signal-regulated kinase (p-ERK), and phosphorylated−mitogen-activated proteinkinase kinase (p-MEK) were confirmed by using western blot analysis. Expressions of p-TYR, p-TRP-1 and p-TRP-2, downstream factors for p-ERK and p-MITF, were translationally inhibited by both extracts. Art.EtOH.FT inducedmore potent effects than Art.EtOH.CT, especially signal transduction effects. In summary, Artemisia capillaris extractsappear to act as potent hypopigmentation agents. KCI Citation Count: 0
ISSN:2466-1384
2466-1392
DOI:10.14405/kjvr.2018.58.1.1