The characteristics of multifocal electroretinogram in isolated perfused porcine eye Cellular contributions to the in vitro porcine mfERG

We investigated characteristics of multifocal electroretinograms (mfERG) from in vitro perfused porcine eyes. TTX, NMDA, APB, and PDA were used to identify contributions to the mfERG from inner retinal neurons, ON-pathway, OFF-pathway, and photoreceptors. The cellular contributions of the first-orde...

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Bibliographic Details
Published inDocumenta ophthalmologica Vol. 117; no. 3; pp. 205 - 214
Main Authors Ng, Yiu-Fai, Chan, Henry H. L., To, Chi-Ho, Yap, Maurice K. H.
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer-Verlag 01.11.2008
Springer
Springer Nature B.V
Subjects
Aminobutyrates
Anesthetics, Inhalation - pharmacology
Anesthetics, Intravenous - pharmacology
Animals
Biological and medical sciences
Electroretinography - drug effects
Excitatory Amino Acid Agonists - pharmacology
Excitatory Amino Acid Antagonists - pharmacology
Isoflurane - pharmacology
Medical sciences
Medicine
Medicine & Public Health
Miscellaneous
N-Methylaspartate - pharmacology
Ophthalmology
Original Research Article
Pipecolic Acids
Propofol - pharmacology
Retina - physiology
Sodium Channel Blockers - pharmacology
Swine
Tetrodotoxin - pharmacology
Multifocal electroretinography
Anesthesia
Retina
Pig
Electroretinography
Characteristic
In vitro
Eye
Electrodiagnosis
Vertebrata
Mammalia
Animal
Artiodactyla
Ophthalmology
Cell
Ungulata
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Summary:We investigated characteristics of multifocal electroretinograms (mfERG) from in vitro perfused porcine eyes. TTX, NMDA, APB, and PDA were used to identify contributions to the mfERG from inner retinal neurons, ON-pathway, OFF-pathway, and photoreceptors. The cellular contributions of the first-order kernel (K1) in an isolated perfused porcine mfERG came from both inner and outer retina, and were similar to those of in vivo porcine mfERG. In addition, application of isoflurane and propofol caused interference with the mfERG response which resembled inner retinal activities sensitive to TTX + NMDA application. Improved understanding of the cellular origins of the perfused porcine mfERG, in the absence of anesthetic agents, is useful for identifying changes shown in the waveform under anesthesia.
ISSN:0012-4486
1573-2622
DOI:10.1007/s10633-008-9124-y