Image analysis of Arabidopsis trichome patterning in 4D confocal datasets
In this article, we present an approach for the automated extraction of quantitative information about trichome patterning on leaves of Arabidopsis thaliana. Time series of growing rosette leaves (4D confocal datasets, 3D + time) are used for this work. At first, significant anatomical structures, i...
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Published in | 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro pp. 742 - 745 |
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Main Authors | , , , , , , |
Format | Conference Proceeding |
Language | English |
Published |
IEEE
01.06.2009
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Subjects | |
Online Access | Get full text |
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Summary: | In this article, we present an approach for the automated extraction of quantitative information about trichome patterning on leaves of Arabidopsis thaliana. Time series of growing rosette leaves (4D confocal datasets, 3D + time) are used for this work. At first, significant anatomical structures, i.e. leaf surface and midplane are extracted robustly. Using the extracted anatomical structures, a biological reference coordinate system is registered to the leaves. The performed registration allows to determine intra- as well as inter-series spatiotemporal correspondences. Trichomes are localized by first detecting candidates using Hough transform. Then, local 3D invariants are extracted and the candidates are validated using a Support Vector Machine (SVM). |
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ISBN: | 1424439310 9781424439317 |
ISSN: | 1945-7928 1945-8452 |
DOI: | 10.1109/ISBI.2009.5193154 |