Triplet-state monitoring by fluorescence correlation spectroscopy

The effects of high excitation intensities in fluorescence correlation spectroscopy (FCS) in terms of saturation and triplet-state build-up have been studied for the case of Rh6G in aqueous solution. It was found that FCS provides a powerful means for the determination of intersystem crossing and tr...

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Bibliographic Details
Published inJournal of fluorescence Vol. 4; no. 3; p. 255
Main Authors Widengren, J, Rigler, R, Mets, U
Format Journal Article
LanguageEnglish
Published Netherlands 01.09.1994
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Summary:The effects of high excitation intensities in fluorescence correlation spectroscopy (FCS) in terms of saturation and triplet-state build-up have been studied for the case of Rh6G in aqueous solution. It was found that FCS provides a powerful means for the determination of intersystem crossing and triplet-state depopulation rates of fluorophores in solution.
ISSN:1053-0509
DOI:10.1007/bf01878460