MUTAGENICITY STUDIES OF PREDNISOLONE FARNESYLATE (PNF)

• Published in: Journal of toxicological sciences Vol. 17; no. SupplementIII; pp. 269 - 281
• Main Authors: OTSUKA, Masanori, AJIMI, Shozo, KAJIWARA, Yoshitsugu, OGURA, Shozo, KAKIMOTO, Keijiro, INAI, Tsunehiko, TANAKA, Hidetsugu, OHUCHIDA, Akinobu
• Format: Journal Article
• Language: English
• Published: Suita The Japanese Society of Toxicology 01.01.1992; Japan Science and Technology Agency
• Subjects: micronucleus test
• ISSN: 0388-1350; 1880-3989
• DOI: 10.2131/jts.17.SupplementIII_269

Prednisolone farnesylate (PNF) was tested for mutagenicity by Ames test using Salmonella typhimurium (TAl00, TA1535, TA98, TA1537) and Escherichia coli (WP2 uvrA), for clastogenic activity in vitro by the chromosomal aberration test in a Chinese hamster fibroblast cell line (CHL), and for induction of micronuclei by the micronucleus test in male ICR mice. 1) In Ames test, PNF with and without metabolic activation showed no mutagenicity in any strains at any dose levels (312∼5, 000 μg/plate). 2) In the chromosomal aberration test, PNF with metabolic activation produced a slight increase in the incidence of structural chromosomal aberrations in CHL cells at l, 500 μg/ml. 3) In the micronucleus test, a single administration of PNF caused no significant increase of micronucleated polychromatic erythrocytes at any doses (250∼2, 000 mg/kg).