An Epitope-Specific Respiratory Syncytial Virus Vaccine Based on an Antibody Scaffold

• Published in: Angewandte Chemie Vol. 127; no. 48; pp. 14739 - 14742
• Main Authors: Luo, Xiaozhou, Liu, Tao, Wang, Ying, Jia, Haiqun, Zhang, Yuhan, Caballero, Dawna, Du, Juanjuan, Wang, Rongsheng E., Wang, Danling, Schultz, Peter G., Wang, Feng
• Format: Journal Article
• Language: English; German
• Published: Weinheim WILEY-VCH Verlag 23.11.2015; WILEY‐VCH Verlag; Wiley Subscription Services, Inc
• Subjects: Affinity; Antibodies; Antikörper; Binding; Chemistry; Immunoglobulins; Konformationen; Neutralizing; Polypeptide; Protein-Engineering; Respiratory diseases; Respiratory syncytial virus; RSV-Vakzin; Scaffolds; Vaccines; Viruses
• ISSN: 0044-8249; 1521-3757
• DOI: 10.1002/ange.201507928

Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract infections in children. We have generated an epitope‐specific RSV vaccine by grafting a neutralizing epitope (F‐epitope) in its native conformation into an immunoglobulin scaffold. The resulting antibody fusion exhibited strong binding affinity to Motavizumab, an RSV neutralizing antibody, and effectively induced potent neutralizing antibodies in mice. This work illustrates the potential of the immunoglobulin molecule as a scaffold to present conformationally constrained B‐cell epitopes. Vakzingerüste durch Antikörper‐Epitop‐Fusion: Durch Bindung eines Virus‐neutralisierenden Epitops (grün) an die komplementaritätsbestimmende Region oder an konstante Schleifen eines Immunglobulins wurden epitopfokussierte Vakzine erhalten.