Biosensors: On-line SERS Detection of Single Bacterium Using Novel SERS Nanoprobes and A Microfluidic Dielectrophoresis Device (Small 22/2014)
On‐line multiplex SERS detection of pathogenic bacteria is demonstrated at a single‐cell level. On page 4700, L.‐K. Chau, C.‐Y. Yang, and co‐workers prepare SERS nanoprobes via immobilization of antibodies on silica‐coated, dye‐induced aggregate beads of gold nanoparticles (AuNPs). The beads give an...
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| Published in | Small (Weinheim an der Bergstrasse, Germany) Vol. 10; no. 22; p. 4414 |
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| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Weinheim
Blackwell Publishing Ltd
01.11.2014
Wiley Subscription Services, Inc |
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| Abstract | On‐line multiplex SERS detection of pathogenic bacteria is demonstrated at a single‐cell level. On page 4700, L.‐K. Chau, C.‐Y. Yang, and co‐workers prepare SERS nanoprobes via immobilization of antibodies on silica‐coated, dye‐induced aggregate beads of gold nanoparticles (AuNPs). The beads give an enhanced Raman signal due to well‐defined plasmonic hot spots at junctions between AuNPs. In combination with dielectrophoresis, they improve the sensitivity of SERS‐barcode biosensing strategies beyond that of whole‐cell ELISA. The practical detection limit over 10 min is 70 CFU mL−1. |
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| AbstractList | On‐line multiplex SERS detection of pathogenic bacteria is demonstrated at a single‐cell level. On page 4700, L.‐K. Chau, C.‐Y. Yang, and co‐workers prepare SERS nanoprobes via immobilization of antibodies on silica‐coated, dye‐induced aggregate beads of gold nanoparticles (AuNPs). The beads give an enhanced Raman signal due to well‐defined plasmonic hot spots at junctions between AuNPs. In combination with dielectrophoresis, they improve the sensitivity of SERS‐barcode biosensing strategies beyond that of whole‐cell ELISA. The practical detection limit over 10 min is 70 CFU mL−1. On-line multiplex SERS detection of pathogenic bacteria is demonstrated at a single-cell level. On page 4700, L.-K. Chau, C.-Y. Yang, and co-workers prepare SERS nanoprobes via immobilization of antibodies on silica-coated, dye-induced aggregate beads of gold nanoparticles (AuNPs). The beads give an enhanced Raman signal due to well-defined plasmonic hot spots at junctions between AuNPs. In combination with dielectrophoresis, they improve the sensitivity of SERS-barcode biosensing strategies beyond that of whole-cell ELISA. The practical detection limit over 10 min is 70 CFU mL super(-1). |
| Author | Kuo, I-Ting Huang, Chen-Han Chau, Lai-Kwan Lin, Hsing-Ying Liu, Ling-Hsuan Lin, Yuan-Chuen Hsieh, Wen-Hsin Wang, Shi-Ting Yang, Chiou-Ying Chu, Chia-Chun |
| Author_xml | – sequence: 1 givenname: Hsing-Ying surname: Lin fullname: Lin, Hsing-Ying organization: Department of Chemistry and Biochemistry and Center for Nano Bio-Detection (AIM-HI), National Chung Cheng University, 62102, Chiayi, Taiwan – sequence: 2 givenname: Chen-Han surname: Huang fullname: Huang, Chen-Han organization: Department of Chemistry and Biochemistry and Center for Nano Bio-Detection (AIM-HI), National Chung Cheng University, 62102, Chiayi, Taiwan – sequence: 3 givenname: Wen-Hsin surname: Hsieh fullname: Hsieh, Wen-Hsin organization: Department of Mechanical Engineering, National Chung Cheng University, 62102, Chiayi, Taiwan – sequence: 4 givenname: Ling-Hsuan surname: Liu fullname: Liu, Ling-Hsuan organization: Department of Chemistry and Biochemistry and Center for Nano Bio-Detection (AIM-HI), National Chung Cheng University, 62102, Chiayi, Taiwan – sequence: 5 givenname: Yuan-Chuen surname: Lin fullname: Lin, Yuan-Chuen organization: Institute of Molecular Biology, National Chung Hsing University, 40227, Taichung, Taiwan – sequence: 6 givenname: Chia-Chun surname: Chu fullname: Chu, Chia-Chun organization: Institute of Molecular Biology, National Chung Hsing University, 40227, Taichung, Taiwan – sequence: 7 givenname: Shi-Ting surname: Wang fullname: Wang, Shi-Ting organization: Department of Chemistry and Biochemistry and Center for Nano Bio-Detection (AIM-HI), National Chung Cheng University, 62102, Chiayi, Taiwan – sequence: 8 givenname: I-Ting surname: Kuo fullname: Kuo, I-Ting organization: Department of Chemistry and Biochemistry and Center for Nano Bio-Detection (AIM-HI), National Chung Cheng University, 62102, Chiayi, Taiwan – sequence: 9 givenname: Lai-Kwan surname: Chau fullname: Chau, Lai-Kwan organization: Department of Chemistry and Biochemistry and Center for Nano Bio-Detection (AIM-HI), National Chung Cheng University, 62102, Chiayi, Taiwan – sequence: 10 givenname: Chiou-Ying surname: Yang fullname: Yang, Chiou-Ying organization: Institute of Molecular Biology, National Chung Hsing University, 40227, Taichung, Taiwan |
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| Snippet | On‐line multiplex SERS detection of pathogenic bacteria is demonstrated at a single‐cell level. On page 4700, L.‐K. Chau, C.‐Y. Yang, and co‐workers prepare... On-line multiplex SERS detection of pathogenic bacteria is demonstrated at a single-cell level. On page 4700, L.-K. Chau, C.-Y. Yang, and co-workers prepare... |
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| SubjectTerms | Bacteria Beads Biosensors Devices Dielectrophoresis Multiplexing Nanostructure Nanotechnology On-line systems raman spectroscopy SERS nanoprobes |
| Title | Biosensors: On-line SERS Detection of Single Bacterium Using Novel SERS Nanoprobes and A Microfluidic Dielectrophoresis Device (Small 22/2014) |
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