Biosensors: On-line SERS Detection of Single Bacterium Using Novel SERS Nanoprobes and A Microfluidic Dielectrophoresis Device (Small 22/2014)

On‐line multiplex SERS detection of pathogenic bacteria is demonstrated at a single‐cell level. On page 4700, L.‐K. Chau, C.‐Y. Yang, and co‐workers prepare SERS nanoprobes via immobilization of antibodies on silica‐coated, dye‐induced aggregate beads of gold nanoparticles (AuNPs). The beads give an...

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Published inSmall (Weinheim an der Bergstrasse, Germany) Vol. 10; no. 22; p. 4414
Main Authors Lin, Hsing-Ying, Huang, Chen-Han, Hsieh, Wen-Hsin, Liu, Ling-Hsuan, Lin, Yuan-Chuen, Chu, Chia-Chun, Wang, Shi-Ting, Kuo, I-Ting, Chau, Lai-Kwan, Yang, Chiou-Ying
Format Journal Article
LanguageEnglish
Published Weinheim Blackwell Publishing Ltd 01.11.2014
Wiley Subscription Services, Inc
Subjects
Bacteria
Beads
Biosensors
Devices
Dielectrophoresis
Multiplexing
Nanostructure
Nanotechnology
On-line systems
raman spectroscopy
SERS nanoprobes
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Summary:On‐line multiplex SERS detection of pathogenic bacteria is demonstrated at a single‐cell level. On page 4700, L.‐K. Chau, C.‐Y. Yang, and co‐workers prepare SERS nanoprobes via immobilization of antibodies on silica‐coated, dye‐induced aggregate beads of gold nanoparticles (AuNPs). The beads give an enhanced Raman signal due to well‐defined plasmonic hot spots at junctions between AuNPs. In combination with dielectrophoresis, they improve the sensitivity of SERS‐barcode biosensing strategies beyond that of whole‐cell ELISA. The practical detection limit over 10 min is 70 CFU mL−1.
Bibliography:istex:C78C5D943137B59DCD1BE4DD37C9F657590CC06E
ArticleID:SMLL201470137
ark:/67375/WNG-8LBGMPX1-R
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SourceType-Scholarly Journals-1
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ISSN:1613-6810
1613-6829
DOI:10.1002/smll.201470137