Chemical reagents for investigating the major groove of DNA

Chemical modification provides an inexpensive and rapid method for characterizing the structure of DNA and its association with drugs and proteins. Numerous conformation-specific probes are available, but most investigations rely on only the most common and readily available of these. The major groo...

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Bibliographic Details
Published inCurrent protocols in nucleic acid chemistry Vol. Chapter 6; p. Unit 6.6
Main Author Rokita, S E
Format Journal Article
LanguageEnglish
Published United States 01.08.2001
Subjects
Base Pairing
Base Sequence
Biochemistry - methods
DNA - chemistry
DNA - genetics
DNA Methylation
DNA Probes - metabolism
G-Quadruplexes
Hot Temperature
Indicators and Reagents
Molecular Sequence Data
Nucleic Acid Conformation
Oxidation-Reduction
Piperidines
Proteins - metabolism
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Summary:Chemical modification provides an inexpensive and rapid method for characterizing the structure of DNA and its association with drugs and proteins. Numerous conformation-specific probes are available, but most investigations rely on only the most common and readily available of these. The major groove of DNA is typically characterized by reaction with dimethyl sulfate, diethyl pyrocarbonate, potassium permanganate, osmium tetroxide, and, quite recently, bromide with monoperoxysulfate. This commentary discusses the specificity of these reagents and their applications in protection, interference, and missing contact experiments.
ISSN:1934-9289
DOI:10.1002/0471142700.nc0606s05