Effect of CO2 and N2 on microbial community changes during column bioleaching of low-grade high pyrite-bearing chalcocite ore
Simulated heap bioleaching of low-grade high pyrite-bearing chalcocite ore was conducted at 40°C with aeration of CO 2 and N 2 . Ore samples were collected at day 43, 64, 85, 106 and subjected to microbial community analysis by 16S rRNA gene clone library. Phylogenetic analyses of 16S rDNA fragments...
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Published in | Journal of Central South University Vol. 22; no. 12; pp. 4528 - 4535 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Changsha
Central South University
01.12.2015
|
Subjects | |
Online Access | Get full text |
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Summary: | Simulated heap bioleaching of low-grade high pyrite-bearing chalcocite ore was conducted at 40°C with aeration of CO
2
and N
2
. Ore samples were collected at day 43, 64, 85, 106 and subjected to microbial community analysis by 16S rRNA gene clone library. Phylogenetic analyses of 16S rDNA fragments revealed that the retrieved sequences are mainly related to genus
Acidithiobacillus
,
Leptospirillum
and
Sulfobacillus
. Aeration of CO
2
and N
2
significantly impacted the microbial community composition. When CO
2
was aerated, the proportion of genus
Acidithiobacillus
considerably increased, whereas the proportion of genus
Leptospirillum
and genus
Sulfobacillus
declined. However, with the aeration of N
2
, the proportion of genus
Acidithiobacillus
and
Leptospirillum
increased, but genus
Sulfobacillus
decreased. When there was no aeration, the microbial community was similar to the inocula with the proportion of genus
Leptospirillum
mounted. These results indicated that the limitation of oxygen could change the bioleaching microbial community and the aeration of CO
2
and N
2
was favourable for the growth of sulfur-oxidizer (
At. caldus
) and iron-oxidizer (
L. ferriphilum
) respectively, which could be used for the regulation of microorganisms’ role in mineral bioleaching. |
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ISSN: | 2095-2899 2227-5223 |
DOI: | 10.1007/s11771-015-3002-z |