The J bs-5YP peptide can alleviate dementia in senile mice by restoring the transcription of Slc40a1 to secrete the excessive iron from brain

• Published in: Journal of advanced research
• Main Authors: Zou, Zhenyou, Wu, Fengyao, Chen, Liguan, Yao, Hua, Wang, Zengxian, Chen, Yongfeng, Qi, Ming, Jiang, Yang, Tang, Longhua, Gan, Xinying, Kong, Lingjia, Yang, Zhicheng, Huang, Xiaolan, Shu, Wei, Li, Bixue, Tan, Xinyu, Huang, Liwen, Bai, Shi, Wu, Lijuan, Mo, Jinping, Hu, Huilin, Liu, Huihua, Zou, Ruyi, Wei, Yuhua
• Format: Journal Article
• Language: English
• Published: Egypt Elsevier B.V 23.03.2024
• Subjects: Alzheimer's Disease; Ets1; Ferroportin; Iron; J bs-5YP Peptide; MWM; AD; BBB; Iron; WB; ATAC; Ets1; Fpn1; ChIP; Ferroportin; Tf; Ftl1; Alzheimer's Disease; CSF; J bs-5YP Peptide; LDLR
• ISSN: 2090-1232; 2090-1224
• DOI: 10.1016/j.jare.2024.03.014

[Display omitted] •ATP level was determined decreased in the body of elderly.•Ets1 was hypo-phosphorylated in the senile mice, weakened the transcription of Slc40a1 and the expression of ferroportin.•J bs-5YP peptide can be absorbed by intestine and cross the Blood-Brain Barrier to enter brain.•J bs-5YP transmits Pi to Ets1 thus resumes Slc40a1 transcription, alleviates iron accumulation in the brain of senile mice. With age and ATP decrease in the body, the transcription factors hypophosphorylation weakens the transcription of Slc40a1 and hinders the expression of the iron discharger ferroportin. This may lead to iron accumulation in the brain and the catalysis of free radicals that damage cerebral neurons and eventually lead to Alzheimer's disease (AD). To prevent AD caused by brain iron excretion disorders and reveal the mechanism of J bs-5YP peptide restoring ferroportin. We prepared J bs-YP peptide and administered it to the senile mice with dementia. Then, the intelligence of the mice was tested using a Morris Water Maze. The ATP content in the body was detected using the ATP hydrophysis and Phosphate precipitation method. The activation of Slc40a1 transcription was assayed with ATAC seq and the ferroportin, as well as the phosphorylation levels of Ets1 in brain were detected by Western Blot. The phosphorylation level of Ets1in brain was enhanced, and subsequently, the transcription of Slc40a1 was activated and ferroportin was increased in the brain, the levels of iron and free radicals were reduced, with the neurons protection, and the dementia was ultimately alleviated in the senile mice. J bs-5YP can recover the expression of ferroportin to excrete excessive iron in the brain of senile mice with dementia by enhancing the transcription of Slc40a1 via phosphorylating Ets1, revealing the potential of J bs-5YP as a drug to alleviate senile dementia.