Measurement of ammonia and glutamine in cell culture media by gas sensing electrodes

This paper describes the use of a commercially available off-line gas sensing electrode for determination of ammonia and glutamine in cell culture media. The measurement technique was tested in different media preparations with different serum concentrations. The glutamine decomposition was studied...

Full description

Saved in:
Bibliographic Details
Published inBiotechnology techniques Vol. 3; no. 4; pp. 217 - 222
Main Authors Ozturk, S.S, Meyerhoff, M.E, Palsson, B.O
Format Journal Article
LanguageEnglish
Published London Chapman and Hall 01.04.1989
Subjects
ammonia
Animal cells
Biological and medical sciences
Biotechnology
cell culture
degradation
electrodes
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
glutamine
hybridomas
metabolism
Methods. Procedures. Technologies
Miscellaneous
sensing
Culture medium
Cell culture
Hybridoma
Metabolism
Assay
Electrodes
Ammonia
Gases
Cell line
Aminoacid
PH
Kinetics
ELISA assay
Glutamine
Online AccessGet full text

Cover

More Information
Summary:This paper describes the use of a commercially available off-line gas sensing electrode for determination of ammonia and glutamine in cell culture media. The measurement technique was tested in different media preparations with different serum concentrations. The glutamine decomposition was studied as a function of pH for cell culture medium and the results were compared to those obtained by conventional methods, i.e., HPLC. Finally, glutamine and ammonia metabolism were studied during the cultivation of a hybridoma cell line.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0951-208X
1573-6784
DOI:10.1007/BF01876051