Oleisolibacter albus gen. nov., sp. nov., isolated from an oil-contaminated soil

• Published in: International journal of systematic and evolutionary microbiology Vol. 69; no. 8; pp. 2220 - 2225
• Main Authors: Ruan, Zhepu, Sun, Qun, Zhang, Yanlin, Jiang, Jian-Dong
• Format: Journal Article
• Language: English
• Published: England 01.08.2019
• Subjects: Rhodospirillaceae; soil; taxonomy
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.003428

A Gram-stain-negative, aerobic, flagellated, rod-shaped bacterium, designated strain NAU-10 , was isolated from an oil-contaminated soil collected in PR China. Strain NAU-10 could grow at 10-42 °C (optimum, 30 °C), pH 5.0-9.0 (pH 7.0) and in the presence of 0-2.5 % (w/v) NaCl (0.5 % in Luria-Bertani broth). The major fatty acids were C18 : 1  ω7c (38.6 %), C17 : 1  ω6c (9.8 %), C18 : 1 2-OH (9.1 %), summed feature 3 (8.7 %), C16 : 0 3-OH (7.2 %) and C16 : 0 (6.7 %). The major respiratory quinones were Q9 and Q10. The total polar lipids were lipid, aminolipid, phospholipid, phosphatidylglycerol and phosphatidylethanolamine. Strain NAU-10 shared the highest 16S rRNA gene sequence similarities with Rhodocista pekingensis 3-p (95.9 %), Niveispirillum cyanobacteriorum TH16 (95.3 %) and Niveispirillum fermenti CC-LY736 (95.3 %), and constituted a sub-cluster within the family Rhodospirillaceae. The DNA G+C content of strain NAU-10 was 68.2 mol% based on its draft genome sequence. Genome annotation of strain NAU-10 predicted the presence of 4309 genes, of which 4237 are coding proteins and 72 are RNA genes. Based on its phenotypic and chemotaxonomic characteristics, as well as the analysis of the 16S rRNA gene sequences, it was concluded that strain NAU-10 represents a novel genus, for which the name Oleisolibacter gen. nov., is proposed. The type species of this genus is Oleisolibacter albus with the type strain NAU-10 (=KCTC 62417 =CCTCC AB 2018015 ).