Determination of Protopine in Rat Brain Tissues by RRLC-ESI/Q-TOF-MS Method

Objective To analyse the quantification of protopine from Corydalis Decumbentis Rhizoma（CDR）extract in brain tissues of rats.Methods A rapid,sensitive,and accurate analytical method based on rapid resolution liquid chromatography（RRLC）coupled with electrospray ionization quadrupole time-of-flight ta...

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Published inChinese herbal medicines Vol. 6; no. 2; pp. 125 - 130
Main Authors Wang, Pei-xiang, Li, Yong-hui, Li, Yan-jing, Geng, Ting, Li, Ming-li, Ding, Gang, Wang, Zhen-zhong, Xiao, Wei
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.05.2014
Subjects
Corydalis Decumbentis Rhizoma
MS法
protopine
RRLC-ESI/ Q-TOF-MS
tissue distribution
串联质谱
大鼠脑
提取物
测定
片碱
相对标准偏差
脑组织
Corydalis Decumbentis Rhizoma
protopine
RRLC-ESI/ Q-TOF-MS
tissue distribution
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Summary:Objective To analyse the quantification of protopine from Corydalis Decumbentis Rhizoma（CDR）extract in brain tissues of rats.Methods A rapid,sensitive,and accurate analytical method based on rapid resolution liquid chromatography（RRLC）coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry（Q-TOF-MS）was developed for the quantification of protopine in brain of rats.A simple liquid-liquid extraction process was employed for the sample preparation.Chromatographic separation was achieved using 1.8μm porous particle columns.Results The calibration curve of protopine was linear in the range of 12-897 ng/mL.The relative standard deviations of intra-and inter-day precision values were less than 10%.The extraction recoveries were 96.4%,99.6%,and 98.5%,for protopine at the concentration of 598.0,119.6,and 12.0 ng/mL,respectively,and internal standard（1.27μg/mL）was（98.60±0.02）%.Conclusion The validated method is successfully applied for the determination of protopine in brain tissue of rats after ig administration of CDR extract.
Bibliography:Corydalis Decumbentis Rhizoma protopine RRLC-ESI/ Q-TOF-MS tissue distribution
12-1410/R
Objective To analyse the quantification of protopine from Corydalis Decumbentis Rhizoma（CDR）extract in brain tissues of rats.Methods A rapid,sensitive,and accurate analytical method based on rapid resolution liquid chromatography（RRLC）coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry（Q-TOF-MS）was developed for the quantification of protopine in brain of rats.A simple liquid-liquid extraction process was employed for the sample preparation.Chromatographic separation was achieved using 1.8μm porous particle columns.Results The calibration curve of protopine was linear in the range of 12-897 ng/mL.The relative standard deviations of intra-and inter-day precision values were less than 10%.The extraction recoveries were 96.4%,99.6%,and 98.5%,for protopine at the concentration of 598.0,119.6,and 12.0 ng/mL,respectively,and internal standard（1.27μg/mL）was（98.60±0.02）%.Conclusion The validated method is successfully applied for the determination of protopine in brain tissue of rats after ig administration of CDR extract.
ISSN:1674-6384
2589-3610
DOI:10.1016/S1674-6384(14)60019-8