Induction of Increased Intracellular Calcium in Astrocytes by Glutamate through Activating NMDA and AMPA Receptors

• Published in: Current medical science Vol. 23; no. 3; pp. 254 - 257
• Main Author: 张蕲 胡波 孙圣刚 童萼塘
• Format: Journal Article
• Language: English
• Published: China 2003
• Subjects: AMPA受体; Animals; Animals, Newborn; Astrocytes - cytology; Astrocytes - metabolism; Biological Transport; Calcium - metabolism; Calcium Channel Blockers - pharmacology; Cells, Cultured; Cytosol - metabolism; Glutamic Acid - pharmacology; Hippocampus - cytology; Hippocampus - metabolism; NMDA受体; Rats; Receptors, AMPA - metabolism; Receptors, N-Methyl-D-Aspartate - metabolism; Synaptic Transmission; 星形胶质细胞; 谷氨酸盐; 钙离子
• ISSN: 1672-0733; 2096-5230; 1993-1352; 2523-899X
• DOI: 10.1007/bf02829506

To study the effect of glutamate on the intraeellular calcium signal of pure cultured rat astroeytes and the role of NMDA and AMPA receptors in the procedure, the change of calcium signal was investigated by monitoring the fluctuation of intraeellular Ca^2+ concentration [(Ca^2+ ]i ) on the basis of Fura-2 single cell fluorescent ratio (F345/F380). The changes in the effect of glutamate on the intracellular calcium signal were observed after blockage of NMDA and(or) AMPA recep-tors. It was found that L-glutamate could induce an increased [(Ca^2+-]i in most of the cells in concen-tration- and time-dependent manner. D-(-)-2-amino-5-phosph0nopentanoic acid (D-AP-5, a selec-tive antagonist of the NMDA receptor) and 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, a selec-tive antagonist of the AMPA receptor) could abolish the effects of NMDA and AMPA respectively.The treatment of D-AP-5 and CNQX simultaneously or respectively could attenuate the effect of Lglutamate at varying degrees. All these indicated that glutamate could modulate intracellular Ca^2+ of pure cultured rat astrocytes through different pathways. The activation of NMDA and AMPA receptors took part in the complex mechanisms.