A novel biosensor for the rapid determination of biochemical oxygen demand

To investigate the function of a novel biosensor used for the rapid determination of biochemical oxygen demand (BOD) which is developed by our research group based on suspended immobilized microbial cell system in a completely mixed determining chamber as a substitute of the traditional membrane sys...

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Bibliographic Details
Published inBiomedical and environmental sciences Vol. 20; no. 1; p. 78
Main Authors Chen, Jin-Song, Zhang, Li-Sheng, Wang, Jian-Long
Format Journal Article
LanguageEnglish
Published China 01.02.2007
Subjects
Biosensing Techniques
Boric Acids - chemistry
Glucose - metabolism
Glutamic Acid - metabolism
Oxygen - analysis
Oxygen - metabolism
Polyvinyl Alcohol - chemistry
Reproducibility of Results
Sewage - microbiology
Temperature
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Summary:To investigate the function of a novel biosensor used for the rapid determination of biochemical oxygen demand (BOD) which is developed by our research group based on suspended immobilized microbial cell system in a completely mixed determining chamber as a substitute of the traditional membrane system. Activated sludge was immobilized by PVA gel and used as a bio-sensing element. The novel biosensor was used to measure the short time BOD value and the conventional cultivation method was used for BOD5 measurement. A linear relationship was observed for the difference between the current and the concentration of glucose-glutamic acid (GGA) solution below 200 mg/L with a correlation coefficient of 0.995. The optimal response of the sensor was obtained at pH 7.0 and 30 degrees C. The sensor response was within 15 min and was reproducible within +/- 5% of the mean in a series of eight samples containing 75 mg/L BOD using standard GGA solution. The novel sensor response was found to be fairly constant over a period of 30 days, with +/- 5% fluctuations. A relatively good agreement is found between BOD estimated by the novel BOD biosensor and that determined by the conventional 5-day BOD method. This novel BOD biosensor has good sensitivity, stability and reproducibility.
ISSN:0895-3988