Assay Methods for the Glycosyltransferases Involved in Synthesis of Bacterial Polysaccharides

Glycans play many important roles in bacterial biology and the complexity of the glycan structures requires biochemical assays in place to help characterize the biosynthetic pathways. Our focus has been on the use of enzymes from pathogens which make molecular mimics of host glycans. We have been ex...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1954; p. 215
Main Authors Abukar, Tasnim, Buenbrazo, Nakita, Janesch, Bettina, Kell, Laura, Wakarchuk, Warren
Format Journal Article
LanguageEnglish
Published United States 2019
Subjects
Lipopolysaccharide
BODIPY
Sialyltransferase
Synthetic acceptor
Enzyme assay
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Summary:Glycans play many important roles in bacterial biology and the complexity of the glycan structures requires biochemical assays in place to help characterize the biosynthetic pathways. Our focus has been on the use of enzymes from pathogens which make molecular mimics of host glycans. We have been examining glycosyltransferases that make strategic linkages in biologically active glycans which can be also exploited for potential therapeutic glycoconjugate synthesis. This chapter will provide details on assays for a variety of bacterial glycosyltransferases that we and others have used for the characterization of pathogen glycoconjugate biosynthetic pathways, and for the in vitro synthesis of human-like glycans produced by bacterial pathogens. The methods presented here should enable other assays to be developed for new pathway characterization.
ISSN:1940-6029
DOI:10.1007/978-1-4939-9154-9_17