Visualization of Trigeminal Ganglion Neuronal Activities in Mice

Visualization of dynamic cellular activity has greatly expanded our understanding of brain function. Recently, there has been an increasing number of studies imaging rodent brain activity in real time. However, traditional in vivo calcium imaging technology has been limited to superficial brain stru...

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Bibliographic Details
Published inCurrent protocols in cell biology Vol. 83; no. 1; p. e84
Main Author Hu, Minghan
Format Journal Article
LanguageEnglish
Published United States 01.06.2019
Subjects
Animals
Brain Mapping - methods
Mice
Neurosurgical Procedures - veterinary
Pain Perception
Trigeminal Ganglion - anatomy & histology
Trigeminal Ganglion - surgery
trigeminal ganglion
GCaMP6
in vivo live imaging
calcium imaging
intravital imaging
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Summary:Visualization of dynamic cellular activity has greatly expanded our understanding of brain function. Recently, there has been an increasing number of studies imaging rodent brain activity in real time. However, traditional in vivo calcium imaging technology has been limited to superficial brain structures. Because the trigeminal ganglion (TG) is located deep within the cranial cavity of mice, few studies have been able to access to it. To circumvent this limitation, overlying brain tissue must be removed to expose the TG so that optical recording can access deep brain neural ensembles. This unit describes a procedure for conducting non-survival surgery to visualize the TG in live mice. Obtaining large ensembles of direct, real-time readouts of sensory neuron signaling, providing temporal and spatial information across the TG, will help to define the cellular basis of orofacial somatic sensing and pain perception. © 2019 by John Wiley & Sons, Inc.
ISSN:1934-2616
DOI:10.1002/cpcb.84