Development and Validation of Multiple Reaction Monitoring (MRM) Assays for Clinical Applications

Selected/multiple reaction monitoring-mass spectrometry (SRM/MRM) is an analytical method that is frequently combined to the use of stable isotope-labeled standard (SIS) peptides for absolute protein quantification. The application of SRM/MRM is a relatively recent development in the proteomics fiel...

Full description

Saved in:
Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1959; p. 205
Main Authors Kontostathi, Georgia, Makridakis, Manousos, Bitsika, Vasiliki, Tsolakos, Nikolaos, Vlahou, Antonia, Zoidakis, Jerome
Format Journal Article
LanguageEnglish
Published United States 2019
Subjects
Plasma
MRM
Quantification
Bioanalytical validation
Proteomics
Online AccessGet more information

Cover

More Information
Summary:Selected/multiple reaction monitoring-mass spectrometry (SRM/MRM) is an analytical method that is frequently combined to the use of stable isotope-labeled standard (SIS) peptides for absolute protein quantification. The application of SRM/MRM is a relatively recent development in the proteomics field for analysis of biological samples (plasma, urine, cell/tissue lysates) targeting to a large extent biomarker validation. Although MRM generally by-passes the use of antibodies (being linked to sub-optimal assay specificity in many cases), bioanalytical validation of MRM protocols has not been robustly applied because of sensitivity issues, in contrary to antibody-based methods. In this chapter, we will discuss the points that should be addressed for MRM method development in clinical proteomics applications.
ISSN:1940-6029
DOI:10.1007/978-1-4939-9164-8_14