Preparation and Characterization of the Magnetic Layered Double Hydroxide - DNA Delivery System

The purpose of this paper is to prepare and evaluate a gene delivery system resulted from the intercalation of DNA with magnetic layered double hydroxide (MLDH). The structure and property of the MLDH/DNA hybrids were studied using XRD, FTIR and TG characterization. The cytotoxicity and protection e...

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Bibliographic Details
Published inMaterials science forum Vol. 914; pp. 3 - 10
Main Authors Sun, Yue, Gou, Guo Jing, Wang, Rui, Yang, Jian Hong, Yao, Hui Qing, Pei, Qin Yu
Format Journal Article
LanguageEnglish
Published Pfaffikon Trans Tech Publications Ltd 01.02.2018
Subjects
Cytotoxicity
Deoxyribonucleic acid
DNA
Electrophoresis
Magnetic properties
Nanoparticles
Toxicity
Nanoparticles
Cytotoxicity
Gene Delivery System
Magnetic Layered Double Hydroxide
Protection against Degradation
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Summary:The purpose of this paper is to prepare and evaluate a gene delivery system resulted from the intercalation of DNA with magnetic layered double hydroxide (MLDH). The structure and property of the MLDH/DNA hybrids were studied using XRD, FTIR and TG characterization. The cytotoxicity and protection effect of MLDH were evaluated by MTT assay and gel electrophoresis assay. MLDH exhibited lower cytotoxicity than other inorganic nanoparticles. Under physiological conditions, MLDH could protect DNA from enzymatic degradation. Our results confirmed that MLDH can be used safely and effectively as non-viral vectors for gene targeting therapy in form of MLDH/DNA composite.
Bibliography:Selected, peer reviewed papers from the 9th China Functional Materials Technology and Industry Forum (9th CEMTIF 2017), August 18-21, 2017, Yinchuan, China
ISSN:0255-5476
1662-9752
1662-9752
DOI:10.4028/www.scientific.net/MSF.914.3