Enhancement of thermal and pH stability of an alkaline metalloprotease by nano-hydroxyapatite and its potential applications

A purified calcium metalloprotease from Bacillus subtilis AKP(N) supplemented with hydroxyapatite nanoparticles [protease (+NP)] showed remarkable increase in thermostability (at 90 °C) over a broad alkaline pH range as compared to the non-supplemented enzyme. Thermodynamic analysis of the protease...

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Bibliographic Details
Published inRSC advances Vol. 5; no. 109; pp. 89346 - 89362
Main Authors Mukhopadhyay, Arka, Chakrabarti, Krishanu
Format Journal Article
LanguageEnglish
Published 01.01.2015
Subjects
Amino acids
Cloth
Detergents
Enzymes
Hydroxyapatite
Nanostructure
Protease
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Summary:A purified calcium metalloprotease from Bacillus subtilis AKP(N) supplemented with hydroxyapatite nanoparticles [protease (+NP)] showed remarkable increase in thermostability (at 90 °C) over a broad alkaline pH range as compared to the non-supplemented enzyme. Thermodynamic analysis of the protease (+NP) showed decreases in K m and activation energy ( E a ) with increased V max and deactivation energy ( E d ) at both 40 °C and 90 °C. The protease (+NP) exhibited compatibility with various laboratory and commercial detergents (1%) at both 40 °C and 90 °C. The protease (+NP) was utilized to efficiently degrade feather to produce amino acids, removal of blood stains from cloth and tenderization of raw meat within a short time.
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ISSN:2046-2069
2046-2069
DOI:10.1039/C5RA16179G