Genes involved in the accumulation of starch and lipids in wheat and rice: characterization using molecular and cytogenetic techniques

This review covers the molecular characterization of starch biosynthetic genes by the Starch group based in CSIRO, Canberra which benefitted enormously from the interactions with Prof Mukai and his group in Osaka. A number of genes involved in starch biosynthesis in wheat were isolated from the D- g...

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Published inNucleus (Calcutta) Vol. 58; no. 3; pp. 185 - 190
Main Authors Muniandy, Kanagesswari, Al-Ajli, Farooq Omar Maan, Ng, Xin Yi, Yazid, Mohd Hafiz Mohd, Tiwari, Gopal Ji, Rahman, Sadequr
Format Journal Article
LanguageEnglish
Published New Delhi Springer India 01.12.2015
Subjects
Biomedical and Life Sciences
Cell Biology
Life Sciences
Review
Malaysia
Biosynthesis
In situ localisation
Oil
Cereals
Grain quality
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Summary:This review covers the molecular characterization of starch biosynthetic genes by the Starch group based in CSIRO, Canberra which benefitted enormously from the interactions with Prof Mukai and his group in Osaka. A number of genes involved in starch biosynthesis in wheat were isolated from the D- genome donor of wheat, Aegilops tauschii. These were usually isolated as lambda clones and therefore their location on chromosomes could only be determined with further work. This gap was filled by the cytogenetic work carried out by Prof Mukai’s group. In addition, when larger BAC clones were isolated, it was often possible by cytogenetics to visualize the organization of multiple genes and to confirm and sometimes correct conclusions from sequencing. New planned work in starch and lipid accumulation at Monash Malaysia is briefly mentioned.
Bibliography:http://dx.doi.org/10.1007/s13237-015-0149-9
ISSN:0029-568X
0976-7975
DOI:10.1007/s13237-015-0149-9